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Vertical transfer of bacteria from dams to neonate calves through colostrum and transition milk ingestion influence the development of the intestinal microbiome, immunity and incidence of diarrhea

Grant number: 17/25569-7
Support type:Research Grants - Visiting Researcher Grant - International
Duration: May 01, 2019 - June 24, 2019
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:Viviani Gomes
Grantee:Viviani Gomes
Visiting researcher: David John Hurley
Visiting researcher institution: University of Georgia (UGA), United States
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The aim of this research will be evaluate the vertical transfer of bacteria by colostrum and milk intake from cows to their offspring, and the influence of this factor on gut microbiome, immunity and diarrhea occurrence in neonate Holstein calves. Dams (n=20) and their offspring from uncomplicated delivery will select for this study. Calves will receive colostrum and transition milk, from their dams, in volume equivalent of 15% of their weight. Fecal samples immediately after delivery (D0), colostrum (D0) and transition milk (D3, D7 and D14) will be harvest from dams. Blood (60mL), fecal samples and rectal mucous will be harvest from neonates on D0, D3, D7 and D14. Mammary secretion will be used to obtain supernatant and cellular fraction, storage at -80°C. The immune response will be evaluated only in the calves. Specific immune response against Staphylococcus aureus, Staphylococcus coagulase-negative (SCN) and Escherichia coli will be evaluated by the following immunological assays: phagocytosis and Reactive Oxygen Species (ROS) production by granulocytes; proliferation, phenotiping and cytokines production from peripheral blood mononuclear cells (PBMC). The plasma and rectal mucus will be used to perform ELISA for specific antibodies (IgG and IgA) for S.aureus, SCN and E.coli. The microbiome of supernatant and cellular fraction from colostrum and transition milk and fecal samples from dams and neonates will be determined by sequencing of 16S rDNA bacterial. The data will be analyzed with appropriate statistical test according to data distribution. (AU)

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VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)