Research Grants 17/21266-0 - Células-tronco, Terapia baseada em transplante de células e tecidos - BV FAPESP
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Amniotic Progenitor cells and therapeutics potention

Grant number: 17/21266-0
Support Opportunities:Regular Research Grants
Start date: April 01, 2018
End date: June 30, 2020
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Carlos Eduardo Ambrósio
Grantee:Carlos Eduardo Ambrósio
Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Associated researchers:Daniele dos Santos Martins ; Silvio Henrique de Freitas ; Valéria Maria Lara Carregaro

Abstract

In recent decades, veterinary medicine and innovative therapies have grown exponentially using new therapeutic tools, either with stem cell use or genetics editions. In this project we focus on studies in undifferentiated cells with high proliferation capacity and fetal characteristics, of the origin of fetal membranes, such as amnion. The amniotic membrane discarded in veterinary medicine is considered an excellent cell source for obtaining stem cells, as it does not require invasive procedures for acquisition and offers a significant amount for preclinical studies. Currently, in veterinary medicine, there is little information related to the immunomodulatory properties of amniotic membrane. Thus, it becomes relevant that these cells are characterized by their potential, immunomodulatory role and secretory components, such as extracellular vesicles. In addition, therapeutic medicine is constantly advancing, and the study of organ development and its molecular functions are in full progression. Another point of study will focus on cell-cell communication through the transport of epigenetic components (microRNA and mRNA) performed by the extracellular vesicles of the amnion, which are secreted by different cell types, the exosomes. We have worked on the creation of an animal source stem cell bank, which could assist different research centers in molecular and clinical understanding and integrate this information. This is one of the proposals in this project, to provide a bank of mesenchymal stem cells from the placental membranes of different animal sources in order to guarantee the safety and quality for the use of these cell lines, establishing free contamination protocols, aiming at the standardization and validation of procedures and analytical techniques involved in the manufacture of cellular therapy products for veterinary use representing an important advance. (AU)

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Scientific publications (12)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MANCANARES, CELINA A. F.; DE OLIVEIRA, VANESSA CRISTINA; OLIVEIRA, LILIAN J.; MIGLINO, MARIA A.; MEIRELLES, FLAVIO VIEIRA; AMBROSIO, CARLOS E.. Morphological and Molecular Analysis of In Vitro Tubular Structures from Bovine Yolk Sac-Derived MSCs. STEM CELLS INTERNATIONAL, . (17/21266-0)
ZOMER, HELENA DEBIAZI; ROBALLO, KELLY C. S.; LESSA, THAIS BORGES; BRESSAN, FABIANA FERNANDES; GONCALVES, NATALIA NARDELI; MEIRELLES, FLAVIO VIEIRA; TRENTIN, ANDREA GONCALVES; AMBROSIO, CARLOS EDUARDO. Distinct features of rabbit and human adipose-derived mesenchymal stem cells: implications for biotechnology and translational research. STEM CELLS AND CLONING-ADVANCES AND APPLICATIONS, v. 11, p. 43-54, . (13/09392-9, 17/21266-0)
DE SOUSA, BARBARA ROSSI; DE OLIVEIRA, VANESSA CRISTINA; PINHEIRO, ALESSANDRA OLIVEIRA; AMBROSIO, CARLOS EDUARDO. Characterization of hematopoietic stem cells from the canine yolk sac. ANIMAL REPRODUCTION, v. 18, n. 2, . (17/21266-0)
KARAM, RAFAEL GARCIA; BRANCO MOTTA, LINA CASTELO; DE ALMEIDA, MATHEUS FERREIRA; BRIDI, ALESSANDRA; DA SILVEIRA, JULIANO COELHO; AMBROSIO, CARLOS EDUARDO. Secretion pattern of canine amniotic stem cells derived extracellular vesicles. ANIMAL REPRODUCTION, v. 19, n. 4, p. 12-pg., . (13/08135-2, 17/21266-0)
TAVARES, M. R.; DE CASTRO, R. V. G.; PIERI, N. C. G.; CRUZ, N. R. N.; MARTINS, D. S.; AMBROSIO, C. E.; GARCIA, J. M.; CAMPLESI, A. C.; BRESSAN, F. F.; TONIOLLO, G. H.. Identification of hepatic progenitor cells in the canine fetal liver. Research in Veterinary Science, v. 133, p. 239-245, . (17/21266-0)
MOTTA, LINA CASTELO BRANCO; PEREIRA, VITORIA MATTOS; PINTO, PRISCILLA AVELINO FERREIRA; MANCANARES, CELINA ALMEIDA FURLANETTO; PIERI, NAIRA CAROLINE GODOI; DE OLIVEIRA, VANESSA CRISTINA; FANTINATO-NETO, PAULO; AMBROSIO, CARLOS EDUARDO. 3D culture of mesenchymal stem cells from the yolk sac to generate intestinal organoid. Theriogenology, v. 209, p. 9-pg., . (17/21266-0)
MANCANARES, CELINA A. F.; DE OLIVEIRA, VANESSA CRISTINA; OLIVEIRA, LILIAN J.; MIGLINO, MARIA A.; MEIRELLES, FLAVIO VIEIRA; AMBROSIO, CARLOS E.. Morphological and Molecular Analysis of In Vitro Tubular Structures from Bovine Yolk Sac-Derived MSCs. STEM CELLS INTERNATIONAL, v. 2019, p. 10-pg., . (17/21266-0)
RAFAEL GARCIA KARAM; LINA CASTELO BRANCO MOTTA; MATHEUS FERREIRA DE ALMEIDA; ALESSANDRA BRIDI; JULIANO COELHO DA SILVEIRA; CARLOS EDUARDO AMBRÓSIO. Secretion pattern of canine amniotic stem cells derived extracellular vesicles. Animal Reproduction, v. 19, n. 4, . (17/21266-0, 13/08135-2)
AMBROSIO, CARLOS EDUARDO; ORLANDIN, JESSICA RODRIGUES; OLIVEIRA, VANESSA CRISTINA; BRANCO MOTTA, LINA CASTELO; FERREIRA PINTO, PRISCILLA AVELINO; PEREIRA, VITORIA MATTOS; PADOVEZE, LETICIA RIBEIRO; KARAM, RAFAEL GARCIA; PINHEIRO, ALESSANDRA DE OLIVEIRA. Potential application of aminiotic stem cells in veterinary medicine. ANIMAL REPRODUCTION, v. 16, n. 1, p. 24-30, . (17/21266-0)
SCASSIOTTI, RODRIGO FERREIRA; COUTINHO, MELINE DE PAULA; SANTOS, SARAH INGRID PINTO; PINTO, PRISCILLA AVELINO FERREIRA; ALMEIDA, MATHEUS FERREIRA; KARAM, RAFAEL GARCIA; ROSA, PAOLA MARIA DA SILVA; MARTINS, DANIELE DOS SANTOS; DA SILVEIRA, JULIANO COELHO; AMBROSIO, CARLOS EDUARDO. Adipose and amnion-derived mesenchymal stem cells: Extracellular vesicles characterization and implication for reproductive biotechnology. Theriogenology, v. 198, p. 9-pg., . (17/21266-0, 22/04928-7, 19/23840-0)
PINHEIRO, ALESSANDRA DE OLIVEIRA; LARA, VALERIA M.; SOUZA, ALINE F.; CASALS, JULIANA B.; BRESSAN, FABIANA F.; FANTINATO NETO, PAULO; OLIVEIRA, VANESSA C.; MARTINS, DANIELE S.; AMBROSIO, CARLOS E.. Characterization and Immunomodulation of Canine Amniotic Membrane Stem Cells. STEM CELLS AND CLONING-ADVANCES AND APPLICATIONS, v. 13, p. 43-55, . (17/21266-0)
ORLANDIN, JESSICA RODRIGUES; GONNES, INGRID DA SILVA; SALLUM LEANDRO, SHAMIRA DE FATIMA; CAGNIM, ARTUR FUERTES; CASALS, JULIANA BARBOSA; CARREGARO, ADRIANO BONFIM; FREITAS, SILVIO HENRIQUE; MACHADO, LUCIANA CRISTINA; REIS CASTIGLIONI, MARIA CRISTINA; GARCIA ALVES, ANA LIZ; et al. Treatment of Chronic Spinal Cord Injury in Dogs Using Amniotic Membrane-Derived Stem Cells: Preliminary Results. STEM CELLS AND CLONING-ADVANCES AND APPLICATIONS, v. 14, p. 39-49, . (17/21266-0, 09/54028-8)