Peptidomics of Brazilian snake and spider venoms

• Grant number: 17/20106-9
• Support Opportunities: Regular Research Grants
• Duration: April 01, 2018 - February 28, 2021
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Alexandre Keiji Tashima
• Grantee: Alexandre Keiji Tashima
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Anita Mitico Tanaka-Azevedo ; Eduardo Shigueo Kitano ; Maria Aparecida Juliano ; Maria Luiza Vilela Oliva ; Solange Maria de Toledo Serrano

Abstract

Venomous animals are amongst the most successful animals on Earth's evolutionary history. One of the important factors for their success is the production of venoms, used to subdue prey and as protection against possible predators. Most active toxins are proteins and peptides, elaborated and refined over millions of years of evolution. Venom toxins have served as leads for molecules with potential pharmacological and biotechnological applications. In addition, they have been important in elucidating biochemical and physiological mechanisms in vertebrates. A landmark in venom research was the discovery of the bradykinin-potentiating peptides, which eventually led to the development of the first drugs for the treatment of arterial hypertension. The development of analytical tools such as next-generation sequencing and mass spectrometers have resulted in the elucidation of thousands of new toxin sequences in recent years. However, despite the large number of sequences discovered, and the maturity achieved by proteomic studies, venom peptidomics is still an emerging research field. Particularities such as the lack of enzymatic specificity in the peptide search and the absence of databases create analytical challenges for such studies. Our group has been working on the improvement of mass spectrometry based analytical techniques applied to the peptidomics, which resulted in the elucidation of several new biologically active venom peptides. We also observed that small mutations have significant effects on the biological activities of peptides within the same class. Given the richness of the still largely unexplored peptidomes venoms, we propose in this project the peptidomic analysis of Brazilian snake and spider venoms with the objectives of unraveling novel peptide sequences, map mutations between homologous sequences and prospect potential biological activities of these peptides. (AU)

Scientific publications

(18)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRICKER, LLOYD D.; TASHIMA, ALEXANDRE K.; FAKIRA, AMANDA K.; HOCHGESCHWENDER, UTE; WETSEL, WILLIAM C.; DEVI, LAKSHMI A.. Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary. Cell Chemical Biology, v. 28, n. 1, p. 105+, JAN 21 2021. (17/20106-9)

DASGUPTA, SAYANI; FISHMAN, MICHAEL A.; CASTRO, LEANDRO M.; TASHIMA, ALEXANDRE K.; FERRO, EMER S.; FRICKER, LLOYD D.. Effect of Protein Denaturation and Enzyme Inhibitors on Proteasomal-Mediated Production of Peptides in Human Embryonic Kidney Cells. BIOMOLECULES, v. 9, n. 6, JUN 2019. (16/04000-3, 17/20106-9)

ZELANIS, ANDRE; SILVA, DEBORA A.; KITANO, EDUARDO S.; LIBERATO, TARCISIO; FUKUSHIMA, ISABELLA; SERRANO, SOLANGE M. T.; TASHIMA, ALEXANDRE K.. A first step towards building spectral libraries as complementary tools for snake venom proteome/peptidome studies. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 31, SEP 2019. (13/14651-3, 14/06579-3, 17/20106-9, 13/07467-1, 17/07897-7, 13/13548-4)

LÍDIA J. TASIMA; CAROLINE SERINO-SILVA; DANIELA M. HATAKEYAMA; ERIKA S. NISHIDUKA; ALEXANDRE K. TASHIMA; SÁVIO S. SANTANNA; KATHLEEN F. GREGO; KAREN DE MORAIS-ZANI; ANITA M. TANAKA-AZEVEDO. Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-04-06. (17/01890-0, 17/16908-2, 14/11108-0, 16/03311-5, 17/20106-9)

ADRIANA SIMIZO; EDUARDO S. KITANO; SÁVIO S. SANTANNA; KATHLEEN FERNANDES GREGO; ANITA MITICO TANAKA-AZEVEDO; ALEXANDRE K. TASHIMA. Comparative gender peptidomics of Bothrops atrox venoms: are there differences between them?. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-10-07. (18/25786-0, 17/21052-0, 17/20106-9, 19/03502-3)

FRITZ, RANI; MUKHERJEE, AMARNATH; ZAGHI, SAHAR; AGALLIU, ILIR; JINDAL, SANGITA; TASHIMA, ALEXANDRE K.; FRICKER, LLOYD D.; DAVIES, KELVIN P.. Identification and characterization of RSIY-11, a novel seminal peptide derived from semenogelin-1, which acts as a neutral endopeptidase inhibitor modulating sperm motility. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, v. 36, n. 9, p. 1891-1900, SEP 2019. (17/20106-9)

HATAKEYAMA, DANIELA MIKI; TASIMA, LIDIA JORGE; BRAVO-TOBAR, CESAR ADOLFO; SERINO-SILVA, CAROLINE; TASHIMA, ALEXANDRE KEIJI; BITTENCOURT RODRIGUES, CAROLINE FABRI; AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; VENANCIO DE LIMA, EDUARDO OLIVEIRA; KAVAZOI, VICTOR KOITI; et al. Venom complexity of Bothrops atrox (common lancehead) siblings. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, OCT 12 2020. (17/16908-2, 18/25786-0, 17/01890-0, 17/20106-9)

AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; NISHIDUKA, ERIKA SAYURI; DE MORAIS-ZANI, KAREN; TANAKA-AZEVEDO, ANITA MITICO. Comparative compositional and functional analyses of Bothrops moojeni specimens reveal several individual variations. PLoS One, v. 14, n. 9, SEP 12 2019. (17/01890-0, 17/16908-2, 14/11108-0, 17/20106-9, 18/20651-0, 18/14724-4, 18/25786-0)

HAYASHI, JACKELINNE Y.; SIMIZO, ADRIANA; MIYAMOTO, JACKSON G.; COSTA, LUCAS V. S.; SOUZA, OLIVIA F.; CHIARELLI, TASSIA; BACAROV, NATALIA B. S.; HIDALGO, RAFAELA; GARCIA, LETICIA D.; SOANE, MICHEL M.; et al. Humoral and cellular responses to vaccination with homologous CoronaVac or ChAdOx1 and heterologous third dose with BNT162b2. Journal of Infection, v. 84, n. 6, p. 3-pg., 2022-06-01. (20/08943-5, 17/20106-9)

HATAKEYAMA, DANIELA MIKI; TASIMA, LIDIA JORGE; GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; FABRI BITTENCOURT RODRIGUES, CAROLINE; STUGINSKI, DANIEL RODRIGUES; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; NISHIDUKA, ERIKA SAYURI; et al. From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages. PLoS One, v. 16, n. 6, JUN 10 2021. (17/16908-2, 17/20106-9, 18/25786-0, 18/25899-0, 18/14724-4)

FRICKER, LLOYD D.; DUARTE, MARIANA LEMOS; JELTYI, ANDREI; LUEPTOW, LINDSAY; FAKIRA, AMANDA K.; TASHIMA, ALEXANDRE K.; HOCHGESCHWENDER, UTE; WETSEL, WILLIAM C.; DEVI, LAKSHMI A.. Mice heterozygous for a null mutation of CPE show reduced expression of carboxypeptidase e mRNA and enzyme activity but normal physiology, behavior, and levels of neuropeptides. Brain Research, v. 1789, p. 12-pg., 2022-08-15. (17/20106-9)

DE MELO, AMANDA TEIXEIRA; MARTHO, KEVIN FELIPE; ROBERTO, THIAGO NUNES; NISHIDUKA, ERIKA S.; MACHADO JR, JOEL; BRUSTOLINI, OTAVIO J. B.; TASHIMA, ALEXANDRE K.; TEREZAVASCONCELOS, ANA; VALLIM, MARCELO A.; PASCON, RENATA C.. The regulation of the sulfur amino acid biosynthetic pathway in Cryptococcus neoformans: the relationship of Cys3, Calcineurin, and Gpp2 phosphatases. SCIENTIFIC REPORTS, v. 9, AUG 15 2019. (16/14542-8, 18/20390-1, 17/20106-9, 15/04400-9)

PINO, JESSICA M. V.; NISHIDUKA, ERIKA S.; DA LUZ, MARCIO H. M.; SILVA, VITORIA F.; ANTUNES, HANNA K. M.; TASHIMA, ALEXANDRE K.; GUEDES, PEDRO L. R.; DE SOUZA, ALTAY A. L.; LEE, KIL S.. ron-deficient diet induces distinct protein profile related to energy metabolism in the striatum and hippocampus of adult rat. NUTRITIONAL NEUROSCIENCE, v. 25, n. 2, MAR 2020. (16/04297-6, 17/20106-9)

VENANCIO DE LIMA, EDUARDO OLIVEIRA; TASIMA, LIDIA JORGE; HATAKEYAMA, DANIELA MIKI; SERINO-SILVA, CAROLINE; BITTENCOURT RODRIGUES, CAROLINE FABRI; GALIZIO, NATHALIA DA COSTA; CHIARELLI, TASSIA; NISHIDUKA, ERIKA SAYURI; TEIXEIRA DA ROCHA, MARISA MARIA; SANT'ANNA, SAVIO STEFANINI; et al. Snake venom color and L-amino acid oxidase: An evidence of long-term captive Crotalus durissus terrificus venom plasticity. Toxicon, v. 193, p. 73-83, APR 15 2021. (18/25786-0, 14/11108-0, 17/01890-0, 16/09976-9, 17/20106-9, 17/21052-0, 17/16908-2, 18/14724-4)

BITTENCOURT RODRIGUES, CAROLINE FABRI; SERINO-SILVA, CAROLINE; DE MORAIS-ZANI, KAREN; KAVAZOI, VICTOR KOITI; NOGUEIRA CARVALHO, MARCELO PIRES; GREGO, KATHLEEN FERNANDES; CHIARELLI, TASSIA; TASHIMA, ALEXANDRE KEIJI; TOYAMA, MARCOS HIKARI; TANAKA-AZEVEDO, ANITA MITICO. Boa.PLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictor. PLoS One, v. 15, n. 2, FEB 27 2020. (17/20106-9, 17/16908-2, 18/25786-0)

NISHIYAMA-JR, MILTON Y.; TASHIMA, ALEXANDRE K.; NISHIDUKA, ERIKA S.; ABREU, THIAGO F.; ABUKAWA, FERNANDA MIDORI; OLIVEIRA, URSULA C.; TARDIVO, CAIO E. O.; NASCIMENTO, SORAIA M.; MEISSNER, GABRIEL O.; CHAIM, OLGA M.; et al. Multiomics Profiling of Toxins in the Venom of the Amazonian Spider Acanthoscurria juruenicola. JOURNAL OF PROTEOME RESEARCH, v. 21, n. 11, p. 15-pg., 2022-10-19. (17/20106-9, 18/13588-0, 13/07467-1)

BARBOZA, BRUNO RAFAEL; THOMAZ, SANDRA MARIA DE OLIVEIRA; DE CARVALHO, AIRTON; ESPREAFICO, ENILZA MARIA; MIYAMOTO, JACKSON GABRIEL; TASHIMA, ALEXANDRE KEIJI; CAMACHO, MAURICIO FROTA; ZELANIS, ANDRE; ROQUE-BARREIRA, MARIA CRISTINA; DA SILVA, THIAGO APARECIDO. ArtinM Cytotoxicity in B Cells Derived from Non-Hodgkin's Lymphoma Depends on Syk and Src Family Kinases. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 2, p. 24-pg., 2023-01-01. (16/04877-2, 18/18538-0, 18/21708-5, 19/05867-9, 17/20106-9)

PINO, JESSICA M. V.; NISHIDUKA, ERIKA S.; DA LUZ, MARCIO H. M.; SILVA, VITORIA F.; ANTUNES, HANNA K. M.; TASHIMA, ALEXANDRE K.; GUEDES, PEDRO L. R.; DE SOUZA, ALTAY A. L.; LEE, KIL S.. Iron-deficient diet induces distinct protein profile related to energy metabolism in the striatum and hippocampus of adult rats. NUTRITIONAL NEUROSCIENCE, v. 25, n. 2, p. 12-pg., 2020-03-18. (17/20106-9, 16/04297-6)