Microelectronic Platforms for Electrochemical, Piezoelectric and FETs Biosensors

• Grant number: 17/24201-6
• Support Opportunities: Regular Research Grants
• Duration: April 01, 2018 - March 31, 2020
• Field of knowledge: Engineering - Electrical Engineering - Electrical, Magnetic and Electronic Measurements, Instrumentation
• Valor Concedido/Desembolsado (R$): 179,259.24 / 179,259.24
• Amount includ. bound (R$): 205,872.33 / 205,872.33
• Principal Investigator: Marcelo Mulato
• Grantee: Marcelo Mulato
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Marina Ribeiro Batistuti ; Pedro Miguel de Lemos Correia Estrela
• Associated grant(s): 18/22155-0 - Dendritic cell interactions for early dengue virus detection and disease understanding, AP.R SPRINT

Abstract

The development of biosensors has been continuously growing every year due tothe possibility of fast and accurate detection of a wide variety of target moleculesor biomarkers, especially in the medical and diagnostic areas. Miniaturization ofthese devices will lead to less invasive testing and reduction of sample volume. Thisproject aims at the study and development of microelectronic biosensors mainlyusing FTO, ITO and gold surfaces modified with polymers, carbon nanostructuresand self-assembled monolayers. Modifications with polymers such as polyanilineallow the immobilization of enzymes and antibodies to detect glucose, lactateand pyruvate. Carbon nanostructures such as graphene and nanotubes allow theimmobilization of enzymes and oligonucleotides. And the gold surfaces can bemodified with polymers as well as with self-assembled monolayers allowing differentmethods of oligonucleotide immobilization, including microRNA and aptamers.The development and optimization of these different platforms, as well as thedetection of the analytes of interest, are based on potentiometric techniques (such aselectrochemical impedance spectroscopy, and extended-gate fieldeffect transistors),piezoelectric (quartz crystal microbalance) and optical methods (through reflectancechange of the polymers after detection). As an innovation, this project startsthe multiplex platform to detect different biomarkers at the same time. Thus, itis expected that, in the end, a further step will be taken in the development ofmicroelectronic biosensors aiming at large-scale medical application, low cost andlow limits of detection. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEDROZA DIAS MELLO, HUGO JOSE NOGUEIRA; BACHOUR JUNIOR, BASSAM; MULATO, MARCELO. Polyaniline-based field effect transistor for DNA/RNA biomarker sensing: Comparison to electrochemical impedance and inorganic layer. SENSORS AND ACTUATORS A-PHYSICAL, v. 318, FEB 1 2021. (17/24201-6, 14/24559-0)

BACHOUR JUNIOR, BASSAM; BATISTUTI, MARINA RIBEIRO; PEREIRA, ALINE SANCHES; DE SOUSA RUSSO, ELISA MARIA; MULATO, MARCELO. Electrochemical aptasensor for NS1 detection: Towards a fast dengue biosensor. Talanta, v. 233, OCT 1 2021. (13/26133-7, 17/24201-6)

FERREIRA, DAISY C.; BATISTUTI, MARINA R.; BACHOUR JUNIOR, BASSAM; MULATO, MARCELO. Aptasensor based on screen-printed electrode for breast cancer detection in undiluted human serum. BIOELECTROCHEMISTRY, v. 137, FEB 2021. (17/13161-3, 13/26133-7, 17/24201-6)

PEDROZA DIAS MELLO, HUGO JOSE NOGUEIRA; MULATO, MARCELO. Enzymatically functionalized polyaniline thin films produced with one-step electrochemical immobilization and its application in glucose and urea potentiometric biosensors. BIOMEDICAL MICRODEVICES, v. 22, n. 1, FEB 27 2020. (14/09562-4, 17/24201-6, 14/24559-0)

MADEIRA, GUSTAVO DANIEL M.; DIAS MELLO, HUGO JOSE N. P.; FALEIROS, MURILO C.; MULATO, MARCELO. Model improvement for super-Nernstian pH sensors: the effect of surface hydration. Journal of Materials Science, v. 56, n. 3, p. 2738-2747, JAN 2021. (18/24395-8, 14/24559-0, 18/22223-5, 17/24201-6)