TISSUE ENGINEERING OF BLOOD VESSELS: MODEL IN RABBITS - IN VITRO

• Grant number: 17/12292-7
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2018
• End date: March 31, 2020
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Matheus Bertanha
• Grantee: Matheus Bertanha
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Elenice Deffune ; Marcone Lima Sobreira ; Patricia Pintor dos Reis
• Associated scholarship(s): 18/08135-6 - Tissue engineering of blood vessels: model in rabbits - in vitro, BP.TT

Abstract

Cardiovascular disease is the leading cause of death in Western populations. The Peripheral Arterial Disease is part of this group of diseases and is present in 5% of the population. In about 20 to 30% of symptomatic cases, evolution occurs for lower limb amputation. Recent research involving stem cells and cell engineering point to a promising future based on replacement therapy and regenerative tissue. Cell therapy is a branch of regenerative medicine and therapeutics and consists of three strands: conductive, which refers to the re-establishment of autonomous cells by external stimuli; inductive, in which growth factors are added to the cell so that it regenerates and the third part is represented by the stem cells, which are used for tissue regeneration therapy. The challenge of this study focuses on the improvement of tissue engineering of blood vessels from animal models (rabbits) in vitro. The technique will be to build and test a new vascular conduit produced from differentiated stem cells. These cells will be induced to differentiate into endothelial tissue after being attached to a decellularized scaffold vena cava, in vitro. The perspective is use this model in the animal in vivo and thereby extended the possibilities of treatment applied in human vascular grafts (bypass), for specific cases where the conventional techniques are not applicable. Thus, it is hoped to contribute for the development of tissue engineering of blood vessels.Keywords: Peripheral Arterial Disease, Stem Cells, Cell Therapy, Endothelium. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PIOVESANA, TADEU RAVAZI; RODRIGUES, LENIZE DA SILVA; DE CARVALHO BOVOLATO, ANA LIVIA; RODRIGUEZ-SANCHEZ, DIEGO NOE; RINALDI, JAQUELINE CARVALHO; SANTOS, NILTON JOSE; MORI, JULIA CALVI; DE ARRUDA LOURENCAO, PEDRO LUIZ TOLEDO; BIRCH, LYNN; BERTANHA, MATHEUS. Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits. BIOMEDICINES, v. 10, n. 11, p. 14-pg., 2022-11-01. (17/12292-7)

SECONDO, MARIANA THAIS SILVA; RODRIGUES, LENIZE DA SILVA; RAMOS, LEANDRO PEREIRA MIRANDA; BOVOLATO, ANA LIVIA CARVALHO; RODRIGUEZ-SANCHEZ, DIEGO NOE; SOBREIRA, MARCONE LIMA; MORAES, MARCELO PADOVANI DE TOLEDO; BERTANHA, MATHEUS. Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering-Experimental Model in Rabbits. BIOMOLECULES, v. 12, n. 12, p. 13-pg., 2022-12-01. (17/12292-7)