Magnetic nanoparticles for biomolecules immobilization

• Grant number: 17/00850-5
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Start date: April 01, 2018
• End date: December 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: William Alberto Leonel Ferreira
• Grantee: William Alberto Leonel Ferreira
• Company: Nchemi Engenharia de Materiais Ltda
• CNAE: Fabricação de aditivos de uso industrial ; Fabricação de produtos químicos não especificados anteriormente
• City: São Carlos
• Associated research grant(s): 18/22473-1 - Magnetic nanoparticles for biomolecules immobilization, AP.PIPE
• Associated scholarship(s): 18/07352-3 - Magnetic nanoparticles for biomolecules immobilization, BP.PIPE

Abstract

The biomedical field demands quick and simple diagnosis along with effective and non-invasive methods. Development of building blocks with specific functions can be the answer to this demand; magnetic nanoparticles (MNPs) have became a promising technology because of its low toxicity and unique magnetic properties. A peculiar class of nanomaterials that can demonstrate an outstanding performance are the so called functionalized nanoparticles. These are particles that present organic molecules bonded onto its surface, i.e., an organic layer. Hence, the MNPs have provided the optimization of regular techniques or the development of totally new ones, such as bioconjugation, magnetic hyperthermia, magnetic resonance imaging and target drug delivery. For these applications, the MNPs requirements include controlled size, high dispersivity and compatibility in biological environment. In this context, this project focus on the development of a method to insert ligands with functional groups responsible for further coupling with biomolecules in the MNPs. The nanoparticles synthesis were developed by the nChemi company. The methodology which perform the best cost/benefit ratio will be further improved. Moreover, the characterization techniques that will be used to analyze the sample quality and characteristics are: XRD, TEM, FT-IR and TGA. The immobilization efficiency will be observed with an LC-MS, in an enzymatic kinetics assay using the enzyme acetylcholinesterase from Electrophorus electricus as standard. The nChemi enterprise group, the Functional Materials Center (CEPID 2013/07296-2) and the chromatography research nucleus (SEPARARE) technically support this project as well. (AU)