Participation of extracellular vesicles of Sporothrix brasiliensis yeast in the immune response against sporothicosis: ex vivo and in vivo studies

• Grant number: 17/26665-0
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2018
• End date: April 30, 2020
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Karen Spadari Ferreira
• Grantee: Karen Spadari Ferreira
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

Sporotrichosis is an emerging mycosis that affects the skin, lymphatic system, and other organs of man and animals. The fungal infection has global distribution and is endemic in some regions of Brazil. The disease is caused by a complex of at least four pathogenic species, including Sporothrix brasiliensis. Some fungi are able to release extracellular vesicles (VEs) to transport molecules, which can interact with the host cell and modulate the immune response. The objective of this work is to analyze the participation of yeasts of S. brasiliensis in the immune response of dendritic cells (DCs) and in the murine model. VEs will be obtained by differential centrifugation from the supernatant of yeast cultures of S. brasiliensis. DCs derived from bone marrow will be stimulated by VEs and then cultured with yeast. Subsequently, the index of phagocytosis, and the fungal load, will be analyzed. In addition, VEs will be inoculated in Balb / c mice prior to subcutaneous infection with the fungus, and the mean diameter and fungal load of the lesion will be analyzed after different times. In this way, we hope to clarify possible virulence factors of the fungus, as well as the modulation of the immune response in vitro and in vivo, using Sporotrhix brasiliensis VEs. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA CAMPOS, RENATO MASSIS; JANNUZZI, GRASIELLE PEREIRA; KAZUO IKEDA, MARCELO AUGUSTO; DE ALMEIDA, SANDRO ROGERIO; FERREIRA, KAREN SPADARI. Extracellular Vesicles From Sporothrix brasiliensis Yeast Cells Increases Fungicidal Activity in Macrophages. Mycopathologia, v. 186, n. 6, SEP 2021. (17/26665-0)