Study of biomolecule mobilization in self-assembled thin films

• Grant number: 07/02295-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2007
• End date: January 31, 2009
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Valtencir Zucolotto
• Grantee: Luana Bendo
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil

Abstract

The advent of techniques based upon the spontaneous assembly of different materials with control over molecular architecture has afforded the fabrication of composite thin films for many nanotechnological applications. This is the case of the Layer-by-layer technique in which materials bearing opposite charges are assembled via electrostatic interactions on solid substrates. This project is aimed at immobilizing biomolecules such as enzymes and nuclear receptors. A detailed investigation of the adsorption process, including kinetics studies, will be carried out. The structural and morphological aspects of the films will be investigated via spectroscopic and microscopic experiments. Parameters such as biological activity and lifetime will be investigated, aiming at the application of the films as biosensors.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BENDO, Luana. Immobilization and sensing ability of human thyroid nuclear receptor in nanostructured thin films. 2010. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.