Enzymatic obtaining of xylitol using Candida guilliermondii xylose reductase as an alternative to fermentative production pathway

• Grant number: 05/02840-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: April 01, 2006
• End date: March 31, 2010
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Silvio Silvério da Silva
• Grantee: Ricardo de Freitas Branco
• Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil

Abstract

Xylitol is a sugar-alcohol with important properties for food, odontological and pharmaceutical industries. It is produced, traditionally, by a chemical process which is very expensive. Fermentative process is a cheaper a alternative which has been extensively studied. However, it results in an inevitable lost of substrate fractions, in different metabolic routes, for biomass and subproducts productions. The enzymatic process is an interesting alternative, which could result in a xylose yield closer to the stoicheometric. The enzymatic production of this polyalcohol could result in a substantial productivity increase, since it wouldn't not have mass transfer flow limitations, usual in microbial process. In this context, the present project has as objective to determinate the conditions of the enzymatic process for xilitol production by xilose redutase from Candida guilliermondii FTI 20037. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRANCO, RICARDO DE FREITAS; SILVA, SILVIO S.. Contribution of Tris Buffer on Xylitol Enzymatic Production. Applied Biochemistry and Biotechnology, v. 162, n. 6, p. 1558-1563, NOV 2010. (05/02840-0)

LALUCE, CECILIA; TOGNOLLI, JOAO OLIMPIO; DE OLIVEIRA, KAREN FERNANDA; SOUZA, CRISLA SERRA; MORAIS, MELINE REZENDE. Optimization of temperature, sugar concentration, and inoculum size to maximize ethanol production without significant decrease in yeast cell viability. Applied Microbiology and Biotechnology, v. 83, n. 4, p. 627-637, June 2009. (05/02840-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BRANCO, Ricardo de Freitas. Xylitol enzymatic production using coenzyme regeneration system as an alternative for the chemical and microbial obtainment way. 2010. Doctoral Thesis - Universidade de São Paulo (USP). Escola de Engenharia de Lorena (EEL/ASDI) Lorena.