Advanced search
Start date
Betweenand

Molecular characterization of cyanobacteria strains isolated from São Paulo State mangrove regions

Grant number: 06/01671-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2007
Effective date (End): June 30, 2010
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Marli de Fátima Fiore
Grantee:Caroline Souza Pamplona da Silva
Home Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

This research project aims to molecular characterize cyanobacterial strains isolated from mangroves regions of the São Paulo State by sequencing their 16S rRNA gene, which has been used to differentiate genetically microorganisms belonging to the Bacteria domain. The sequences obtained will be compared by BLAST (NCBI) analysis and the evolutionary distance between the sequenced nucleotides strains will be analyzed by phylogenetic tree construction. At least fifty cyanobacterial strains will be evaluated. The PCR amplification of sequences of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) enzymes genes will allow to identify among the molecular characterized strains those potentially producing of biochemically active natural compound (cyanopeptides). These enzymes are known to be involved in toxins and antibiotics biosynthesis. The detection of these nonribosomal peptides genes will be carried out using degenerate oligonucleotides primers target to conserved sequences of NRPS and PKS enzymes. The PCR products obtained for these enzymes will be sequenced. These sequences also will be compared by BLAST analysis and their evolutionary relations will be verified by phylogenetic tree construction. The cyanopeptides produced by the strains containing NRPS and PKS genes will be extracted using several solvents. The chemical constituent of cellular extracts will be analyzed using Q-TOF-MS (Quadrupole Time-of-Flight Mass Spectrometry) and HPLC (High Performance Liquid Chromatography). The inhibitory activity of these chemical compounds will be tested against fungus and bacteria.

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PAMPLONA SILVA, CAROLINE SOUZA; GENUARIO, DIEGO BONALDO; MARCAL VIEIRA VAZ, MARCELO GOMES; FIORE, MARLI FATIMA. Phylogeny of culturable cyanobacteria from Brazilian mangroves. Systematic and Applied Microbiology, v. 37, n. 2, p. 100-112, MAR 2014. Web of Science Citations: 29.
SILVA-STENICO, MARIA ESTELA; LORENZI, ADRIANA STURION; TESCHKE, OMAR; PAMPLONA SILVA, CAROLINE SOUZA; ETCHEGARAY, AUGUSTO; FIORE, MARLI FATIMA. Antimicrobial Cyanopeptide Action on Bacterial Cells Observed with Atomic Force Microscopy. CURRENT NANOSCIENCE, v. 9, n. 1, p. 141-148, FEB 2013. Web of Science Citations: 1.
SILVA-STENICO, MARIA ESTELA; VIEIRA, FELIPE D. P.; GENUARIO, DIEGO B.; SILVA, CAROLINE S. P.; MORAES, LUIZ ALBERTO B.; FIORE, MARLI FATIMA. Decolorization of Textile Dyes by Cyanobacteria. Journal of the Brazilian Chemical Society, v. 23, n. 10, p. 1863-1870, OCT 2012. Web of Science Citations: 7.
SILVA-STENICO, MARIA ESTELA; PAMPLONA SILVA, CAROLINE SOUZA; LORENZI, ADRIANA STURION; SHISHIDO, TANIA KEIKO; ETCHEGARAY, AUGUSTO; LIRA, SIMONE POSSEDENTE; BERALDO MORAES, LUIZ ALBERTO; FIORE, MARLI FATIMA. Non-ribosomal peptides produced by Brazilian cyanobacterial isolates with antimicrobial activity. MICROBIOLOGICAL RESEARCH, v. 166, n. 3, p. 161-175, 2011. Web of Science Citations: 34.
FIORE, MARLI FATIMA; GENUARIO, DIEGO BONALDO; PAMPLONA DA SILVA, CAROLINE SOUZA; SHISHIDO, TANIA KEIKO; BERALDO MORAES, LUIZ ALBERTO; CANTUSIO NETO, ROMEU; SILVA-STENICO, MARIA ESTELA. Microcystin production by a freshwater spring cyanobacterium of the genus Fischerella. Toxicon, v. 53, n. 7-8, p. 754-761, JUN 2009. Web of Science Citations: 38.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.