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RANKL and osteoprotegerin expression in gingival fluid and tissue in different peri-implantitis severity before and after anti-infection therapy: a study in human beings

Grant number: 06/04604-4
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2007
Effective date (End): February 28, 2009
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Poliana Mendes Duarte
Grantee:Vanessa Renata Santos da Silva
Home Institution: Universidade de Guarulhos (UNG). Campus Guarulhos-Centro. Guarulhos , SP, Brazil


This study will evaluate the “receptor activator of NF-{kappa}B ligand" (RANKL) e osteoprotegerin (OPG) expression in different forms of perimplantitis before and after mechanic anti- infection therapy. Forty dental implants will be divided in four groups: Group 1: mucositis; Group 2: light to moderate perimplantitis; Group 3: severe perimplantitis; Group 4: health perimplant tissue. It will be only included implants with indication for surgical procedures (groups 2 and 3: surgical access flap/ groups 1 and 4: gingival hyperplasia). RANKL and OPG gene and protein expressions will be analyzed by quantitative PCR and ELISA test respectively. Gingival biopsies will be colleted and mRNA levels analyzed by quantitative PCR. Crevicular fluid will be collected at the beginning of the study and 90 days after treatments for ELISA test. Intergroup analysis will be done by ANOVA and intragroup analysis by Repeated Measures ANOVA (alpha= 0.05) for ELISA results. “Real Time” PCR data will be evaluated by one way ANOVA (alpha= 0.05). (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DUARTE, POLIANA MENDES; DE MENDONCA, ADRIANA CUTRIM; BRAZ MAXIMO, MARIA BEATRIZ; SANTOS, VANESSA RENATA; BASTOS, MARTA FERREIRA; NOCITI JUNIOR, FRANCISCO HUMBERTO. Differential cytokine expressions affect the severity of peri-implant disease. Clinical Oral Implants Research, v. 20, n. 5, p. 514-520, MAY 2009. Web of Science Citations: 56.
DE MENDONCA, ADRIANA CUTRIM; SANTOS, VANESSA RENATA; CESAR-NETO, JOAO BATISTA; DUARTE, POLIANA MENDES. Tumor Necrosis Factor-Alpha Levels After Surgical Anti-infective Mechanical Therapy for Peri-Implantitis: A 12-Month Follow-Up. Journal of Periodontology, v. 80, n. 4, p. 693-699, APR 2009. Web of Science Citations: 19.
DUARTE, POLIANA MENDES; DE MENDONCA, ADRIANA CUTRIM; BRAZ MAXIMO, MARIA BEATRIZ; SANTOS, VANESSA RENATA; BASTOS, MARTA FERREIRA; NOCITI, JR., FRANCISCO HUMBERTO. Effect of Anti-Infective Mechanical Therapy on Clinical Parameters and Cytokine Levels in Human Peri-Implant Diseases. Journal of Periodontology, v. 80, n. 2, p. 234-243, FEB 2009. Web of Science Citations: 57.

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