Analysis of antimicrobial activity of purified fractions and extracts of marine invertebrates and microorganisms on Streptococcus mutans and influence on their virulence factors

• Grant number: 07/01078-2
• Support type: Scholarships in Brazil - Master
• Effective date (Start): December 01, 2007
• Effective date (End): February 28, 2009
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Reginaldo Bruno Gonçalves
• Grantee: Bruna de Araujo Lima
• Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

Streptococcus mutans is the main etiologic agent of the dental caries. The synthesis of lactic acid, the acid tolerance and the formation of dental biofilm by glucosiltransferases enzymes and glucan binding proteins, are virulence factors of this microorganism involved in the process of development of the dental caries. The search for new antimicrobial natural agents capable to inhibit such mechanisms of virulence or to control the growth of pathogenic microorganisms, may represent an important strategy for prevention and treatment of this infection. Recent studies had shown that natural products of sea origin (mainly proceeding from marine invertebrates and microorganisms) possess significant antimicrobial activity against antibiotics resistant pathogenic microorganisms.The objective of this study will be analyze the antimicrobial activity of extracts or purified fractions obtained from marine invertebrates and microorganisms on several oral streptococci strains of medical and dental importance and S. mutans isolates. Additionally, the study will evaluate the activities of extract and/or purified fractions on virulence factors of S. mutans (acid production, acid tolerance and biofilm formation). The results will be able to select new natural substances with anticariogenic / cariostatic potential.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, BRUNA DE A.; DE LIRA, SIMONE P.; KOSSUGA, MIRIAM H.; GONCALVES, REGINALDO B.; BERLINCK, ROBERTO G. S.; KAMIYA, REGIANNE U. Halistanol sulfate A and rodriguesines A and B are antimicrobial and antibiofilm agents against the cariogenic bacterium Streptococcus mutans. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 24, n. 6, p. 651-659, NOV-DEC 2014. Web of Science Citations: 6.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LIMA, Bruna de Araujo. Analysis of antimicrobial activity of purified fractions and extracts of marine invertebrates and microorganisms on Streptococcus mutans and influence on main virulence factors. 2009. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba.