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Differential phospho and glyco proteomic analysis in gastric adenocarcinoma and the eventual correlation with clinic-pathological parameters of prognosis

Grant number: 07/02470-3
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2007
Effective date (End): April 30, 2011
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Marilia de Arruda Cardoso Smith
Grantee:Mariana Ferreira Leal
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Gastric cancer is the 4th most frequent type of cancer and is the second most common cause of cancer death in the World. Brazil is in 4th place concerning higher prevalence of gastric cancer in Latin America and Pará State presents a high incidence of this neoplasia. The etio-pathology of gastric carcinogenesis gastric is still fragmented. The modification of this panorama may rely on the detection of protein alterations in tumor development, which would allow an accurate approach for prognosis, diagnosis and therapeutic management. Few studies on protein expression, glycosylation and phosphorylation profiles in primary gastric cancer samples were done. In the present study, proteic expression, glycosylation and phosphorylation profiles will be investigated in 24 gastric cancer and 24 normal gastric tissue samples. To study the protein expression profile, samples will be separate by bidimensional gel electrophoresis and proteins sub or superexpressed will be identified by mass spectrometry. Glycosylation and phosphorylation profiles will be evaluated by Pro-Q® 300 Emerald glycoprotein stain and Pro-Q® Diamond Phosphoprotein Gel Stain respectively. Proteins with difference in glycosylation and phosphorylation status will be identified by mass spectometry. These profiles will be compared considering presence or absence of neoplasia, presence or absence of Helicobacter pylori, clinical stage, gastric adenocarcinoma type and differentiation degree, as well as presence or absence of lymph node metastases. Proteins found with superexpression in neoplastic samples will be evaluated by Western Blotting in 50 serum samples of gastric cancer patients and 50 of volunteers, aiming investigate circulating diagnostic markers and a less invasive assay. All samples will be collected in University Hospital João of Barros Barreto of Pará State. This study will generate new and additional biological informations to provide etio and physiopathology understanding of this neoplasia with potential therapeutic use.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
WISNIESKI, FERNANDA; GERALDIS, JAQUELINE CRUZ; SANTOS, LEONARDO CAIRES; LEAL, MARIANA FERREIRA; CALCAGNO, DANIELLE QUEIROZ; GIGEK, CAROLINA OLIVEIRA; CHEN, ELIZABETH SUCHI; ANAUATE, ANA CAROLINA; ARTIGIANI, RICARDO; DEMACHKI, SAMIA; ASSUMPCAO, PAULO PIMENTEL; LOURENCO, LAERCIO GOMES; ARASAKI, CARLOS HARUO; KRAINER, JULIE; PABINGER, STEPHAN; BURBANO, ROMMEL RODRIGUEZ; CARDOSO SMITH, MARILIA ARRUDA. Differential regulation of LRRC37A2 in gastric cancer by DNA methylation. Epigenetics, FEB 2021. Web of Science Citations: 0.
COELHO MOREIRA, CARLOS ONETE; FERREIRA BORGES DA COSTA, JOANA DE FATIMA; LEAL, MARIANA FERREIRA; DE ANDRADE, EDILSON FERREIRA; REZENDE, ALEXANDRE PINGARILHO; IMBELONI, ALINE AMARAL; PEREIRA CARNEIRO MUNIZ, JOSE AUGUSTO; CARDOSO SMITH, MARILIA DE ARRUDA; BURBANO, ROMMEL RODRIGUEZ; DE ASSUMPCAO, PAULO PIMENTEL. Lymphocyte proliferation stimulated by activated Cebus apella macrophages treated with a complex homeopathic immune response modifiers. Homeopathy, v. 101, n. 1, p. 74-79, JAN 2012. Web of Science Citations: 8.
FERREIRA BORGES DA COSTA, JOANA DE FATIMA; LEAL, MARIANA FERREIRA; RAIOL SILVA, TANIELLY CRISTINA; ANDRADE JUNIOR, EDILSON FERREIRA; REZENDE, ALEXANDRE PINGARILHO; PEREIRA CARNEIRO MUNIZ, JOSE AUGUSTO; CUNHA LACRETA JUNIOR, ANTONIO CARLOS; ASSUMPCAO, PAULO PIMENTEL; CALCAGNO, DANIELLE QUEIROZ; DEMACHKI, SAMIA; BAREM RABENHORST, SILVIA HELENA; CARDOSO SMITH, MARILIA DE ARRUDA; BURBANO, ROMMEL RODRIGUEZ. Experimental gastric carcinogenesis in Cebus apella nonhuman primates. PLoS One, v. 6, n. 7, p. e21988, 2011. Web of Science Citations: 13.

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