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EVALUATION OF GENOTOXIC AND MUTAGENIC EFFECTS OF COMMERCIAL BIODIESEL AND DIESEL BLENDS PURE AND IN SPILL SIMULATIONS IN WATER AND SOIL

Grant number: 07/04304-3
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2007
Effective date (End): November 30, 2010
Field of knowledge:Biological Sciences - Pharmacology - Toxicology
Principal Investigator:Maria Aparecida Marin Morales
Grantee:Daniela Morais Leme
Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil

Abstract

The exhaustion of the energy sources, mainly of fossil energy, has been motivated the development of new technologies based on renewable energies. Biodiesel is, nowadays, an outstanding fuel, since it is derived from renewable sources, as vegetables and animal oils. The biodiesel uses has some advantages under diesel fuel, as reduction of air pollution and its rapid degradability in the environment. However, the uses of only this fuel in diesel engines are still problematic, so it has been commercialized in blends with diesel fuel. In the literature, only studies about the impacts caused by the use of diesel and biodiesel blends to air pollution were founded, but studies of the impacts to other biotic systems - water and soil - and to exposed organisms were not carried out yet. Considering the necessity of protection health of living organism and to assure the ecosystem quality, this project aimed to analyze the genotoxic and mutagenic effects of diesel and biodiesel blends, which are widely used due to the commercialization imposed by law. The tests will be carried out using pure blends and in cases of leakages, by the spill simulations of diesel and biodiesel blends in water and soil. For that, the test system uses will be Salmonella typhimurium, Allium cepa and HepG2 cells. Chromosome aberration in the meristematic cells and the micronucleus in F1 cells will be evaluated in the Allium test. For the HepG2 cells will be applied the comet assay and the micronucleus assay. The choice of such test systems were determined, based on their sensitivity for mutagens detection, as well as the necessity of evaluations the health effects caused to different living organisms groups, since the environmental contamination is concern not only to humans, but also to all ecosystem.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LEME, DANIELA MORAIS; GRUMMT, TAMARA; DE OLIVEIRA, DANIELLE PALMA; SEHR, ANDREA; RENZ, SYLVIA; REINEL, SISSY; FERRAZ, ELISA R. A.; RODRIGUES DE MARCHI, MARY ROSA; MACHADO, MARCOS CANTO; ZOCOLO, GUILHERME JULIAO; MARIN-MORALES, MARIA APARECIDA. Genotoxicity assessment of water soluble fractions of biodiesel and its diesel blends using the Salmonella assay and the in vitro MicroFlow (R) kit (Litron) assay. Chemosphere, v. 86, n. 5, p. 512-520, FEB 2012. Web of Science Citations: 15.
LEME, DANIELA MORAIS; GRUMMT, TAMARA; HEINZE, RITA; SEHR, ANDREA; RENZ, SYLVIA; REINEL, SISSY; DE OLIVEIRA, DANIELLE PALMA; FERRAZ, ELISA R. A.; RODRIGUES DE MARCHI, MARY ROSA; MACHADO, MARCOS CANTO; ZOCOLO, GUILHERME JULIAO; MARIN-MORALES, MARIA APARECIDA. An overview of biodiesel soil pollution: Data based on cytotoxicity and genotoxicity assessments. JOURNAL OF HAZARDOUS MATERIALS, v. 199, p. 343-349, JAN 15 2012. Web of Science Citations: 12.
LEME, DANIELA MORAIS; GRUMMT, TAMARA; HEINZE, RITA; SEHR, ANDREA; SKERSWETAT, MATTHIAS; RODRIGUES DE MARCHI, MARY ROSA; MACHADO, MARCOS CANTO; DE OLIVEIRA, DANIELLE PALMA; MANN-MORALES, MARIA APARECIDA. Cytotoxicity of water-soluble fraction from biodiesel and its diesel blends to human cell lines. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 74, n. 8, p. 2148-2155, NOV 2011. Web of Science Citations: 14.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LEME, Daniela Morais. Avaliação da genotoxicidade e mutagenicidade de misturas comerciais de diesel e biodiesel puras e em simulações de vazamento em água e solo. 2010. 207 f. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Biociências (Campus de Rio Claro). Rio Claro.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.