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Application of the radiation in the inhibition of alimentary allergy the shrimp(Litopenaeus vannamei)

Grant number: 08/08182-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2009
Effective date (End): December 31, 2011
Field of knowledge:Agronomical Sciences - Food Science and Technology
Principal Investigator:Solange Guidolin Canniatti Brazaca
Grantee:Tatiana Giselle Guimarães Lopes
Home Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

The hypersensitivity to foods is very common in children and adults. The shrimp in spite of very appreciated is a food potentially capable to unchain allergic reactions genetically in individuals per disposed. The major allergen in shrimp is the tropomiosin. The objective of that experiment is to evaluate the effects of the radiation in the reduction of the alimentary allergy to shrimp, making possible consumption for intolerant individuals. The samples will be constituted of shrimps in natura and freeze-dry conditioned in trays of polystyrene, wrapped in flexible plastic film and submitted to different radiation doses (5,0; 10,0 and 15,0 kGy), being those maintained under cooling (5ºC ± 1ºC). They will be produced at laboratory the antibodies that will be used in the experiment to evaluate the alergenicidade of the protein tropomiosin. The samples, later to the irradiation, they will go by analyses for tropomiosin determination using the techniques: SDS-Page, HPLC, ELISA and Imunobloting. Acceptance test will be done to verify physic-chemical and sensorial attributes after irradiation. Datas will be evaluating by independent assays, randomized. Datas will be evaluated by ANOVA and Tukey test (pd0,05).

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LOPES, Tatiana Giselle Guimarães. Efeito da radiação gama na reatividade alergênica e nas propriedades físico-químicas e sensoriais de camarão (Litopenaeus vannamei). 2012. Doctoral Thesis - Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura Piracicaba.

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