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Using of color Doppler and Power Doppler as predictive factor and their correlation with immunohistochemical markers in canine mast cell tumor

Grant number: 08/10164-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2009
Effective date (End): April 30, 2010
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Renee Laufer Amorim
Grantee:Sabrina dos Santos Costa
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Carcinogenesis is a process developed in several phases, the angiogenesis is known as an essencial phase for tumor progression and metastasis. The aim of the proposed study is evaluate vascularization of mast cell tumors and correlate it with dog's prognosis. The research will be performed in 20 dogs with mast cell tumor, of any histopathological grade. After diagnosis confirmation of mast cell tumor by fine needle aspirates, dogs wil be held in the research and anamnesis and physical exam will de done. The nodules with be measured with pachymeter and the lymph nodes will be punctured to look for metastasis. The assessment of tumor vascularity will be done by ultrasound, using Doppler tecnology with a 10MHZ linear transducer to evaluate the vascularization with color Doppler and Power Doppler. After surgical removal of the nodules, the histopathological graduation according to Patnaick et al (1984) and immunoistochemical study will be performed with the primary antibodies anti-HIF-1a, anti-fator VIII, c-Kit, MIB-1 and caspase-3. Tumor location, metastasis, ulceration, time evolution of the disease and survival in those dogs will be correlated to the histopathological grade, vascular density demonstrated by Doppler and by the number of positive vessels for VIII factor. Datas as imunoexpression to HIF-1 will be correlated with vascular density in Doppler and immunohistochemical study.

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
COSTA, Sabrina dos Santos. Utilização de Doppler como fator prognóstico e suas correlações com marcadores imunoistoquímicos no mastocitoma cutâneo canino. 2011. 65 f. Doctoral Thesis - Universidade Estadual Paulista. Faculdade de Medicina Veterinária e Zootecnia. Botucatu.

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