In vitro Immune Response to Antigens of Human Papillomavirus (HPV) in Men of Sao Paulo, Brasil

• Grant number: 09/00347-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2009
• Effective date (End): October 31, 2012
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Jorge Simao do Rosario Casseb
• Grantee: Fernando Augusto Miranda da Costa
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Objective: To evaluate the specific immune response in vitro to HPV in men with lesions caused by HPV and without injury caused by HPV. Methods: We recruited 31 patients and 11 volunteers, who formed four groups: 12 patients in Group A (HIV+/HPV+); 09 patients in Group B (HIV-/HPV+); 10 patients in Group C (HIV+/HPV-) and 11 healthy subjects in Group D (HIV-/HPV-). Cells culture assay was performed to measure the specific immune response "in vitro" Th1/Th2/Th17 (IFN-³, IL-2, TNF-±, IL-4, IL-10 and IL-17) under the stimulation of quadrivalent HPV vaccine (HPV 6, 11, 16 and 18) and the E7 protein of HPV-16. Results: The coinfected group (HIV+/HPV+) had higher levels of cytokines, especially Th2 cytokines, compared with data from the others study groups. The coinfected group showed high levels of IL-6 and IL-10 (Th2 profile) compared to the Control Group (HIV-/HPV-), with statistical significance (p<0.0001 and p<0.0001, respectively). Conclusion: This study demonstrated a high production of cytokines in the coinfected group, suggesting a strong immunomodulation by coinfection HIV/HPV. However, further studies should be conducted to confirm these data. In addition to presenting essentially a Th2 profile, especially by high levels of IL-6 and IL-10 presented, suggesting that these two cytokines may serve as biomarkers for viral persistence, since HIV seropositive patients have a higher persistence of HPV, and monitor the progression to more serious injuries.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

COSTA, Fernando Augusto Miranda da. In vitro immune response to antigens of human papillomavirus (HPV) in men of Sao Paulo, Brasil. 2013. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.