Role of P2 receptors in the cell differentiation and cell death of osteoblasts

• Grant number: 09/02775-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2009
• End date: July 31, 2011
• Field of knowledge: Biological Sciences - Biophysics - Cellular Biophysics
• Principal Investigator: Alice Teixeira Ferreira
• Grantee: Renata Rodrigues
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Mechanical movements during the exercise (non-lyti liberation) and fractures (lytic liberation) cause liberation of the molecules such as ATP in osseous tissue. Liberation ATP can activate receptors on the cellular membrane (P2 or purinergic receptors) which accelerate the process of bone formation. The P2 receptors are subdivided in P2X (ion channels) and P2Y (G protein coupled), and are expressed in most tissues, including the osseous tissue. Thus, in recent years, has studied the role P2 receptors in the growth of bones and the repair of fractures, showing the presence of some functional subtypes of P2 receptors, suggesting the involvement of P2X receptors in the proliferation of osteoblasts, and P2Y receptors in differentiation, however no direct evidence of these assumptions is doing further studies to clarify the real function of P2 receptors in these processes. Furthermore, recent results obtained by our group have showed the action of ATP in osteoblasts by mechanical stimuli such as ultrasound; parallel, we observed that high concentrations of ATP and UTP promote reduction in the number of cells by mechanisms not yet described. Thus, this project aims to determine the role of P2 receptors in the process of differentiation and cell death of osteoblasts assisting in understanding the physiological role of these receptors in bone tissue helping to clarify the role of P2 receptors in this osseous tissue.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES-RIBEIRO, RENATA; ALVARENGA, ERIKA COSTA; CALIO, MICHELE LONGONI; PAREDES-GAMERO, EDGAR JULIAN; FERREIRA, ALICE TEIXEIRA. Dual Role of P2 Receptors during Osteoblast Differentiation. Cell Biochemistry and Biophysics, v. 71, n. 2, p. 1225-1233, MAR 2015. (09/02775-4)