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The role of conditioned medium of mesenchymal stem cells on apoptose process of proximal tubular cells induced by hypoxia

Grant number: 09/06416-9
Support type:Scholarships in Brazil - Master
Effective date (Start): September 01, 2009
Effective date (End): August 31, 2011
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Niels Olsen Saraiva Câmara
Grantee:Flavia D Angelo Maculan
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Associated research grant:07/07139-3 - The role of heme oxygenase 1 in different renal inflammatory process in experimental animal models, AP.TEM

Abstract

Acute kidney injury is closely related with ischemia and toxic injury. Reperfusion triggers a worsening into this injury due to inflammation caused by an increase of reactive oxygen species (ROS), leading to cellular death by necrosis and apoptosis. Thus, alternative therapies are necessary to revert or prevent this panorama. In this context, the adult stem therapies are rising, since they may repair/regenerate injury tissue. The stem cell´s action mechanism is unknown; however several works suggest that they may repair the tissue directly, by repopulating the tissue or indirectly by the secretion of trophic factors. In fact, the latter is more accepted nowadays. In this work, we will analyze if the trophic factors secreted by adult stem cell, by using a conditioned medium of mesenchymal stem cells (MSC), are capable to rescue renal proximal tubular cells of apoptosis induced by hypoxia in vitro. We will use MSC and HK-2 (immortalized proximal tubular cell) and, we intend to evaluate the profile of apoptosis, oxidative stress, pro-inflammatory cytokines and reactive species of oxygen (ROS) in these cells submitted to hypoxia in vitro, in the presence or not of conditioned medium. We intend to demonstrate that the regenerative role of the MSC involves the secretion of trophic factors, presented in the culture medium, and furthermore that those factors would present pro-survival properties