Study of structural proteins of Anticarsia gemmatalis nucleopoliedrovirus (AgMNPV-2D) in different insect cell lines and comparison of the GP64 in geographic variants

• Grant number: 09/16740-8
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: May 01, 2010
• End date: April 30, 2013
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Paolo Marinho de Andrade Zanotto
• Grantee: Carla Torres Braconi
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Since the 80's many Latin American countries and the United States have been using the multiple nucleopolyhedrovirus Anticarsia gemmatalis (AgMNPV) as a biological control to the velvetbean caterpillar (A. gammatalis).In Brazil there are at least one million hectares of soybean plantation that uses this viral control with great ecological and economical benefits for the country. The AgMNPV also plays an important whole as a clone vector and eucaryotic gene expressor. This virus is a enveloped one, it belongs to the Baculoviridae family with a circular double DNA strand surrounded by a proteic capsid, that can be evolved by a paracristalline matrix. Due to its great commercial and biotechnological importance, this virus has been studied in many ways. Our laboratory coordinated the AgMNPV-2D complete genome sequencing, finding 152 potential "ORFs", with 24 ORFs coding for structural proteins. One of this, is the glycoprotein 64 (GP64) of the BV phenotype of NPV I. The GP64 is the main protein involved in the secondary infection of the multiple nucleopolyhedrovirus Anticarsia gemmatalis. In AgMNPV the GP64 function dominions are distinct from the others members of NPVI group. As a natural consequence of the sequencing, my project has the following main goals: (i) determine and characterize part of AgMNPV-2D structural protein sequence; (ii) identify different expressed proteins from the two viral phenotypes (ODV and BV), in distinct lepidopteran line cells; (iii) map in silico the "ORFs" responsible for the structural protein expression from both viral phenotypes and (iv) identify the difference between GP64 from geographic variants of AgMNPV, leading, thus to a better comprehension about the viral morphogenesis and the associated factors of the viral multiplication. Therefore, the follow methods will be applied: SDS page, 2D electrophoresis, mass spectrometry and cloning and sequencing gp64. This study will allow a better comprehension about the viral morphogenesis and the associated factors of the viral multiplication. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRACONI, CARLA TORRES; PEREIRA ARDISSON-ARAUJO, DANIEL MENDES; PAES LEME, ADRIANA FRANCO; DE CASTRO OLIVEIRA, JULIANA VELASCO; PAULETTI, BIANCA ALVES; GARCIA-MARUNIAK, ALEJANDRA; RIBEIRO, BERGMANN MORAIS; MARUNIAK, JAMES E.; DE ANDRADE ZANOTTO, PAOLO MARINHO. Proteomic analyses of baculovirus Anticarsia gemmatalis multiple nucleopolyhedrovirus budded and occluded virus. JOURNAL OF GENERAL VIROLOGY, v. 95, n. 4, p. 980-989, APR 2014. (08/10713-6, 09/16740-8)

BRACONI, CARLA TORRES; PEREIRA ARDISSON-ARAUJO, DANIEL MENDES; PAES LEME, ADRIANA FRANCO; DE CASTRO OLIVEIRA, JULIANA VELASCO; PAULETTI, BIANCA ALVES; GARCIA-MARUNIAK, ALEJANDRA; RIBEIRO, BERGMANN MORAIS; MARUNIAK, JAMES E.; DE ANDRADE ZANOTTO, PAOLO MARINHO. Proteomic analyses of baculovirus Anticarsia gemmatalis multiple nucleopolyhedrovirus budded and occluded virus. JOURNAL OF GENERAL VIROLOGY, v. 95, p. 10-pg., 2014-04-01. (09/16740-8, 08/10713-6)

IAMARINO, ATILA; DE MELO, FERNANDO LUCAS; BRACONI, CARLA TORRES; DE ANDRADE ZANOTTO, PAOLO MARINHO. BF Integrase Genes of HIV-1 Circulating in Sao Paulo, Brazil, with a Recurrent Recombination Region. PLoS One, v. 7, n. 4, APR 2 2012. (09/16740-8, 07/01554-9, 10/19341-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BRACONI, Carla Torres. The baculovirus Anticarsia gemmatalis multiple nucleopolyhedrovirus proteome and the comparison of multiple isolated envelope proteins GP64.. 2013. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.