Penicillin G is inactivated by stomach acid pH, which is why it is used in intravenous. Penicillins are distributed in various tissues, and difficulty of crossing the blood-brain barrier, are not biotransformed in the body, being eliminated by the kidneys, 90% by tubular secretion and 10% by glomerular filtration. This project aims to describe and analyze the collagen, elastic and smooth muscle fibers of the renal pelvis of Wistar rats by analyzing structural changes in experimental groups compared with the control group regarding the use of penicillin G. The mice will be divided into 4 different groups (A, B, C and D): normal rats, rats treated with penicillin G, rats induced diabetes, diabetic rats with penicillin G. The rats in groups C and D will be induced to diabetes by alloxan. The region of the renal pelvis that contains representation of fibers will be collected and reduced into small fragments. The cuts obtained are stained by the following methods: Iron hematoxylin: for demonstration of elastic; Resorcin fuchsin: for demonstration of elastic fibers and elastic fibers; Resorcin fuchsin after oxidation with aqueous 1% oxone: for demonstration of elastic fibers, elastic fibers and elastic fibers; Azan for disclosure of the component collagen and smooth muscle; Picrosirius to observe the component collagen, specifically collagens type I and III; Hematoxylin and eosin, to show the cellular component; Weigert-Van Gieson, for disclosure and disposition of the elastic structure inside the collagen tissue and smooth muscle. The results will be analyzed according to each technique. Macroscopic analysis and imaging will allow the understanding of changes in the renal pelvis of rats in the study, allowing to make comparisons between the control and experimental groups. The primary focus of attention in general is related to the differences caused by penicillin G in experimental mice. Light microscopy and scanning electron microscopy and transmission will allow the understanding of the biology of cellular and tissue specimens.
News published in Agência FAPESP Newsletter about the scholarship: