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Evaluation of genetic and biochemical biomarkers in Nile Tilapia (Oreochromis niloticus) exposed to herbicides combine (tebutiurom) and Velpar (diurom + hexaninona)

Grant number: 10/04028-9
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2010
Effective date (End): January 31, 2012
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Eduardo Alves de Almeida
Grantee:Mariana Furio Franco Bernardes
Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil


The aquatic environment is invariably struck by xenobiotics (chemical of natural or man-made). Herbicides are a heterogeneous category of chemicals, made specifically for weed control, which can reach aquatic ecosystems. These xenobiotics can be grouped according to the mechanism of action, including inhibitors of photosystem II of photosynthesis. Among these are the possessors of chemical groups triazines (herbicide atrazine), triazinones (herbicide hexazinone) and urea (herbicides diurom and tebutiurom). Biomarkers can be defined as a body changes that are indicative of exposure or effect of a xenobiotic. Several cellular components in fish have been used as biomarkers, including the analysis of the genetic and biochemical parameters. In order to analyze the genotoxic, mutagenic and metabolic herbicide Combine ® (tebutiurom) and Velpar ® (diurom + hexazinone) in Nile tilapia (Oreochromis niloticus) are examined, as genetic biomarkers, the frequency of micronuclei and nuclear abnormalities in addition to the comet assay. As biochemical biomarkers will be analyzed the levels of the activities of biotransformation enzymes 7-etóxiresorufina-O-desetilase (EROD) and glutathione S-transferases (GST), the antioxidant enzyme superoxide dismutase (SOD) and lipid peroxidation (LPO).