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Sanitary surveillance of bovine encephalitis: bovine leukaemia virus as causative agent

Grant number: 10/04692-6
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2010
Effective date (End): January 31, 2012
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Claudia Del Fava
Grantee:Rubens Henrique Ramos D'Angelino
Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The Laboratory of anatomopathology of Instituto Biologico/SP is authorized by the Brazilian Ministry of Agriculture (MAPA) to perform the histopathological differential diagnosis of encephalitis and Bovine Spongiform Encephalopathy. From January 2004 to June 2007 were examined by histopathology 690 brains from cattle with neurological syndrome negative to rabies (direct immunofluorescence), 40.0% (276) presented lesions, and the non purulent meningoencephalitis was the most frequent - 90.0% (249/276). This inflammatory infiltrate characterized by mononuclear cells indicates that viruses, parasites or bacterias are causing encephalitis, and that is necessary to use specific and sensitive tests to diagnose then. The present study proposes to diagnose Bovine Leukaemia Virus in bovine brain samples sent to Instituto Biológico perform the differential diagnosis of neurological syndrome, by the association of lesions by histology (Hematoxilin/Eosin stainning) and PCR in cattle negative for rabies. The genomic sequencing of the BLV will be performed in order to verify the genetic polymorphism of the samples obtained. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RAMOS D'ANGELINO, RUBENS HENRIQUE; PITUCO, EDVIGES MARISTELA; CASSARO VILLALOBOS, ELIANA MONTEFORTE; HARAKAVA, RICARDO; GREGORI, FABIO; DEL FAVA, CLAUDIA. Detection of Bovine Leukemia Virus in Brains of Cattle with a Neurological Syndrome: Pathological and Molecular Studies. BIOMED RESEARCH INTERNATIONAL, . (11/06513-4, 10/04692-6)
RAMOS D'ANGELINO, RUBENS HENRIQUE; PITUCO, EDVIGES MARISTELA; CASSARO VILLALOBOS, ELIANA MONTEFORTE; HARAKAVA, RICARDO; GREGORI, FABIO; DEL FAVA, CLAUDIA. Detection of Bovine Leukemia Virus in Brains of Cattle with a Neurological Syndrome: Pathological and Molecular Studies. BIOMED RESEARCH INTERNATIONAL, v. 2013, p. 6-pg., . (10/04692-6, 11/06513-4)

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