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Development of rapid methods for sample preparation and chemical speciation of arsenic in foods by LC-ICP-MS and evaluation of metabolism and concentration in rice

Grant number: 10/07566-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2010
Effective date (End): June 30, 2012
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Fernando Barbosa Júnior
Grantee:Bruno Lemos Batista
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


The chemical elements, typically set by physico-chemical properties in solid form, can be divided in essential and toxic. The essential elements are those needed to maintain its normal physiological functions such as Zn, Fe, Co, Mg, Se. The toxic elements are those that have no function in the body such as Cd, Pb, Hg and As. An example, arsenic (As) is widely distributed in the environment being considered by the ATSDR (USA) the most toxic compound to humans. It is linked to several peripheral vascular diseases, heart, anemia and cancer. Inorganic forms, As(III) and As(V), are more toxic, being methylated to methylarsonic acid (MMA) and dimethylarsinic acid (DMA). This methylation is not constant, varying according to age, gender, ethnicity, exposure dose, pregnancy, nutritional status and genetic polymorphism, causing variations in the toxicity of the species. In humans the As-compounds are employed as medicaments for the treatment of leukemia. In animals as growth and antiparasitic drugs and, finally, they can be used as pesticides. Thus, due to their high water solubility, oral exposure is more common. Countries such as Japan, USA, Australia and the European Community are developing policy efforts (laws) and technology to evaluate the concentration of species of compounds in food (imported) and the environment. Thus, due to the increasing demands of domestic and international markets for food safety, it is essential to develop quick and simple methods for routine application in speciation analysis by HPLC-ICP-MS. Abroad, many research groups use the HPLC-ICP-MS for As speciation because it is a versatile tool with high sensitivity and selectivity. Several methods of extraction and quantification of As species in matrices such as liver, kidneys, blood, serum, urine, hair, apple, rice and marine organisms have been developed. However, in Brazil, a important food exporter, there is a deficiency in the speciation analysis of As. Therefore, this project aims to: i) develop and validate a method for separation and quantification of As(III), As(V), DMA, MMA and Arsenobetaína HPLC-ICP-MS; ii) develop a simple extraction of the species for application in routine; iii) apply the method in rice and fish samples commonly consumed in Brazil for food safety; iv) study the As uptake and metabolism of As by rice plants (Oryza sativa L.).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BATISTA, BRUNO LEMOS; NIGAR, MEHER; MESTROT, ADRIEN; ROCHA, BRUNO ALVES; BARBOSA JUNIOR, FERNANDO; PRICE, ADAM H.; RAAB, ANDREA; FELDMANN, JOERG. Identification and quantification of phytochelatins in roots of rice to long-term exposure: evidence of individual role on arsenic accumulation and translocation. Journal of Experimental Botany, v. 65, n. 6, p. 1467-1479, . (10/07566-1)
FREIRE, BRUNA MOREIRA; SANTOS, VIVIAN DA SILVA; FERREIRA NEVES, PERICLES DE CARVALHO; OLIVEIRA SOUZA REIS, JULIANA MARIA; DE SOUZA, SAMUEL SIMIAO; BARBOSA JR, FERNANDO; BATISTA, BRUNO LEMOS. Elemental chemical composition and As speciation in rice varieties selected for biofortification. ANALYTICAL METHODS, v. 12, n. 16, p. 2102-2113, . (17/20914-8, 10/07566-1, 14/05151-0)
BORGES, ENDLER MARCEL; LAFAYETTE NEVES GELINSKI, JANE MARY; DE OLIVEIRA SOUZA, VANESSA CRISTINA; BARBOSA, JR., FERNANDO; BATISTA, BRUNO LEMOS. Monitoring the authenticity of organic rice via chemometric analysis of elemental data. Food Research International, v. 77, n. 3, p. 299-309, . (12/03746-0, 12/22663-9, 12/03465-1, 10/07566-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BATISTA, Bruno Lemos. Development of rapid methods for sample preparation and chemical speciation of arsenic in foods by LC-ICP-MS and evaluation of metabolism and concentration in rice. 2012. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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