Identification of producing IL-10-regulatory B cells induced by T. nattereri venom immunization

• Grant number: 10/15913-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2010
• End date: October 31, 2012
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Carla Lima da Silva
• Grantee: Erica Maria Martins Coutinho
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The contribution of B cells for the immunological memory is based on two different populations: memory B cells and long-lived antibodies secreting cells (ASC). The interrelation among these cells as well as the mechanisms involved in their maintenance has been little understood. Functional B cell regulation of immunopathology has been established in EAE, chronic intestinal inflammation and systemic lupus erythematosis. The venom of Thalassophryne nattereri possesses the ability to induce an intense memory immune response. The results showed that venom promoted splenomegaly accompanied by intense cell proliferation and germinal centers formation for 120 days after immunization and induced high and constant production of specific antibodies as IgG1, IgG2a and IgE anaphylactic. Furthermore, we observed an expansion of B1a cells in the peritoneum and spleen of immunized animals and we identify a distinct cell memory responders that emerge rapidly upon 21 days after Ag rechallenge and persist at different levels into compartments (peritoneum, spleen and bone marrow). Here the main objective of this work is the investigation the emergence of IL-10-producing regulatory B (Breg) cells during murine model of memory response.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRUNI, FERNANDA MIRIANE; MARTINS COUTINHO, ERICA MARIA; ANDRADE-BARROS, ALINE INGRID; GRUND, LIDIANE ZITO; LOPES-FERREIRA, MONICA; LIMA, CARLA. Anaphylaxis induced by Thalassophryne nattereri venom in mice is an IgE/IgG1-mediated, IL-4-dependent phenomenon. SCIENTIFIC REPORTS, v. 10, n. 1, JAN 17 2020. (10/15913-3)