Sugarcane (Saccharum spp) is an economically important grass once it constitutes the raw material for products like sugar and ethanol. Proteomics is a science that complements the results obtained by transcriptomics and metabolomics. There are proteomic studies found in several plant species, but little has been done with regard to sugarcane. Besides, the subproteome is advantageous because it improves the sensibility and resolution of the method, reducing the complexity of the proteome analyzed. The knowledge of the nuclear proteome is also extremely important, once the genetic material and the machinery necessary for its duplication and transcription are localized inside the nucleus. This knowledge is essential for the comprehension of gene regulation and expression mechanisms. Furthermore, studies found in different plant species demonstrate that most of the nuclear proteins are related to these processes. It has also been verified that in sugarcane 44% of the proteins are localized in the nucleus. Thus, the study of nuclear proteins of leaves in different development stages will help in the comprehension of regulation mechanisms, and will also provide data for the improvement of agronomically important characteristics.
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