Carbaryl (1-naphthyl methylcarbamate), a broad spectrum insecticide widely used in agriculture and domestic environment, has recently been associated with the development of cutaneous melanoma in an epidemiological cohort study with U.S. farm workers. Parallelly, ultraviolet radiation is known to be the main etiologic factor for skin cancer development. Although comprehensive and well designed, the epidemiological study does not allow to characterize the direct contribution of the insecticide and radiation in melanomagenesis. Several studies have explored the synergistic effect of certain chemicals to UV radiation, increasing its deleterious effects on the skin, and possibly contributing to tumor development. For example, enzymes cytochrome P450 1A1 and 1B1 are hyperexpressed in human skin after exposure to UV light, and this condition is likely to intensify bioactivation of environmental chemical carcinogens, increasing the risk of skin cancer development. This study aims to evaluate the influence of the insecticide carbaryl on the effects of UV radiation on cultured human melanocytes, assessing biotransformation parameters, cytotoxicity, genotoxicity and melanocyte activation.
News published in Agência FAPESP Newsletter about the scholarship: