Activation profile of neutrophils from elderly individuals and expression of IgG receptors

• Grant number: 10/19317-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2011
• End date: January 31, 2012
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Thaís Helena Gasparoto
• Grantee: Thalita Marcato Dalboni
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Neutrophils are the first cells to migrate to infections site and they perform important role in the defense against several pathogens. The function of neutrophils involves phagocytosis and destruction of microrganisms through reactive oxygen species, antimicrobian enzymes and peptides; besides cytokines and chemokines production in order to attract and activate other defense cells. Neutrophils express membrane receptors for linking opsonized or not pathogens, and they are able to trigger intracellular activation cascades culminating in neutrophils effective response against infections. Receptors of the IgG class immunoglobulins (CD16, CD32 and CD64) execute fundamental role in the recognition of opsonized microrganisms, and they are expressed at different activation stages of neutrophils, according to the factors of the microenvironment, specially the cytokine/chemokine interleucin 8 (IL-8). Neutrophils present phenotypic and functional changes with the advance of the age. Such changes may directly affect their activation and survival, facilitating establishment and persistence of diseases. The aim of this work is to evaluate the pattern of IgG receptors expression on neutrophils and possible alterations related to the ageing. Therefore, we will analyze induced CD16, CD32 and CD64 expression, survival, enzymatic activity and cytokines production for neutrophils, obtained of elderly and young subjects, after in vitro stimulated by IL-8 and bacterial lipopolysaccharyde (LPS). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ABE, ANELI E.; DE OLIVEIRA, CARINE E.; DALBONI, THALITA M.; CHAGAS-PAULA, DANIELA A.; ROCHA, BRUNO A.; DE OLIVEIRA, REJANE B.; GASPAROTO, THAIS H.; DA COSTA, FERNANDO B.; CAMPANELLI, ANA P.. Anti-inflammatory sesquiterpene lactones from Tithonia diversifolia trigger different effects on human neutrophils. REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 25, n. 2, p. 111-116, MAR-APR 2015. (08/02185-0, 11/01862-0, 11/18174-0, 09/14127-7, 10/10940-2, 10/19317-6)

GASPAROTO, THAIS HELENA; DALBONI, THALITA MARCATO; AMOR, NADIA GHINELLI; ABE, ANELI EIKO; PERRI, GRAZIELA; LARA, VANESSA SOARES; VIEIRA, NARCISO ALMEIDA; GASPAROTO, CARLOS TEODORO; CAMPANELLI, ANA PAULA. Fc gamma receptors on aging neutrophils. Journal of Applied Oral Science, v. 29, 2021. (09/14127-7, 10/19317-6)

DALBONI, THALITA MARCATO; ABE, ANELI EIKO; DE OLIVEIRA, CARINE ERVOLINO; LARA, VANESSA SOARES; CAMPANELLI, ANA PAULA; GASPAROTO, CARLOS TEODORO; GASPAROTO, THAIS HELENA. Activation profile of CXCL8-stimulated neutrophils and aging. CYTOKINE, v. 61, n. 3, p. 716-719, MAR 2013. (10/19317-6)