GENOTOXICITY AND MUTAGENICITY ASSESSMENT OF REACTIVE DYE EXTRACTED OF COTTON FIBERS WITH ARTIFICIAL SWEAT

• Grant number: 10/14941-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): March 01, 2011
• Effective date (End): August 31, 2014
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Danielle Palma de Oliveira
• Grantee: Daniela Morais Leme
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Abstract:Human eyes have a remarkable ability to recognize hundreds of color shades, which has stimulated the development and commercialization of dyes and pigments. Several natural and synthetic dyes are nowadays available to use in dyeing process, especially for clothing. Toxicological studies have shown that several kinds of textile dyes can be hazardous to human health. It is know that conditions of intense perspiration generate a high rate of migration and subsequent penetration of textile dyes to human skin. This is a relevant fact, since humans are frequently exposed to dyes potential hazardous to their health. The cotton clothes are the most commonly used in Brazil, due to the weather conditions (high temperatures) of our country. Different dye groups can be used in dyeing cotton fibers, however, reactive dyes have been outstanding in this process. Dye molecular structure is divided in two parts: cromophere group responsible for the color and the part responsible for the fixation to the fiber. For reactive dyes, covalent bound between the fibers and the groups clorotriazin and sulfatoethilsulfone allow the fixation. Taking into account the problem mentioned herein, the present study aim to evaluate genotoxic and mutagenic effects of a reactive dye extracted of cotton fiber with artificial sweat. For this propose, different methods will be applied as following: Salmonella/microsoma assay to detect point mutation; genotoxicity and mutagenicity assessment using human skin fibroblasts in vitro cell culture and 3D skin model (Episkin®). Key-Words: cotton fiber dyeing; reactive dye; artificial sweat; DNA damage; Salmonella/microsoma assay; comet assay; MN test; human skin fibroblasts and Episkin®.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEME, DANIELA MORAIS; RODRIGUES DE OLIVEIRA, GISELE AUGUSTO; MEIRELES, GABRIELA; BRITO, LARA BARROSO; RODRIGUES, LAIS DE BRITO; DE OLIVEIRA, DANIELLE PALMA. Eco- and Genotoxicological Assessments of Two Reactive Textile Dyes. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 78, n. 5, p. 287-300, MAR 4 2015. (10/14941-3)

LEME, DANIELA MORAIS; RODRIGUES DE OLIVEIRA, GISELE AUGUSTO; MEIRELES, GABRIELA; DOS SANTOS, TUANE CRISTINA; BOLDRIN ZANONI, MARIA VALNICE; DE OLIVEIRA, DANIELLE PALMA. Genotoxicological assessment of two reactive dyes extracted from cotton fibres using artificial sweat. TOXICOLOGY IN VITRO, v. 28, n. 1, SI, p. 31-38, FEB 2014. (10/14941-3)

LEME, DANIELA MORAIS; PRIMO, FERNANDO LUCAS; GOBO, GRACIELY GOMIDES; VIEIRA DA COSTA, CLEBER RAFAEL; TEDESCO, ANTONIO CLAUDIO; DE OLIVEIRA, DANIELLE PALMA. Genotoxicity Assessment of Reactive and Disperse Textile Dyes Using Human Dermal Equivalent (3D Cell Culture System). JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v. 78, n. 7, p. 466-480, APR 3 2015. (10/14941-3)

LEME, DANIELA MORAIS; RODRIGUES DE OLIVEIRA, GISELE AUGUSTO; MEIRELES, GABRIELA; DOS SANTOS, TUANE CRISTINA; BOLDRIN ZANONI, MARIA VALNICE; DE OLIVEIRA, DANIELLE PALMA. Genotoxicological assessment of two reactive dyes extracted from cotton fibres using artificial sweat. TOXICOLOGY IN VITRO, v. 28, n. 1, p. 8-pg., 2014-02-01. (10/14941-3)