Abstract:Human eyes have a remarkable ability to recognize hundreds of color shades, which has stimulated the development and commercialization of dyes and pigments. Several natural and synthetic dyes are nowadays available to use in dyeing process, especially for clothing. Toxicological studies have shown that several kinds of textile dyes can be hazardous to human health. It is know that conditions of intense perspiration generate a high rate of migration and subsequent penetration of textile dyes to human skin. This is a relevant fact, since humans are frequently exposed to dyes potential hazardous to their health. The cotton clothes are the most commonly used in Brazil, due to the weather conditions (high temperatures) of our country. Different dye groups can be used in dyeing cotton fibers, however, reactive dyes have been outstanding in this process. Dye molecular structure is divided in two parts: cromophere group responsible for the color and the part responsible for the fixation to the fiber. For reactive dyes, covalent bound between the fibers and the groups clorotriazin and sulfatoethilsulfone allow the fixation. Taking into account the problem mentioned herein, the present study aim to evaluate genotoxic and mutagenic effects of a reactive dye extracted of cotton fiber with artificial sweat. For this propose, different methods will be applied as following: Salmonella/microsoma assay to detect point mutation; genotoxicity and mutagenicity assessment using human skin fibroblasts in vitro cell culture and 3D skin model (Episkin®). Key-Words: cotton fiber dyeing; reactive dye; artificial sweat; DNA damage; Salmonella/microsoma assay; comet assay; MN test; human skin fibroblasts and Episkin®.
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