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Effect of resistive training on peripehral insulin sensitivity and pro and anti-inflammatory markers in skeletal muscle from obese rats

Grant number: 10/17078-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2011
Effective date (End): December 31, 2011
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Patricia Monteiro Seraphim
Grantee:Rafael Junges Moreira
Home Institution: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil

Abstract

Obesity is manifested by the pandemic, affecting millions of people, mainly in Western and industrialized countries. Metabolism disorders often related to obesity contribute to the dysfunction of multiple organs and systems. In obese individuals, the expansion of adipose tissue, organ reserve of energy, increases production and release of pro-inflammatory adipokine with effects. Among these, the most studied and is known as TNF-alpha, which can activate another inflammatory pathway NFkB / IKKb engaged role in insulin resistance during obesity. However, it is noteworthy that in addition to adipose tissue, skeletal muscle also produces and releases cytokines in response to contraction that can positively influence the homeostasis glicidic site. The micro-trauma to muscle tissue induced by exercise stimulates the production of cytokines with anti-inflammatory effects, such as IL-10 for restriction occur that the magnitude and duration of the inflammatory response after exercise. Furthermore, the physical exercise reduces the content of TNF-alpha and also inhibits the inflammatory pathway NFkB / IKKb with promotion of improved insulin signaling. Therefore, this study aims to evaluate whether the anaerobic training can ease the inflammation site and improve insulin sensitivity in obese mice by cafeteria diet, the main predictors of mRNA content of TNF-alpha, IL-10 in tissues and NFkB muscle. Will be used male Wistar rats divided into four groups: Sedentary Control (SC) Exercise Control (EC) Sedentary Obese (SO); Exercise Obese (EO). Control animals are fed standard chow and water ad libitum, the animals of the SO and EO groups will be fed a hypercaloric diet (cafeteria diet) adapted LAMAS (2004) from the second month of life. The anaerobic training will be held three times a week for six weeks, following the model of force-type "squat jump" proposed by Tetsuro TAMAKI et al. 1992, with some adaptations. An electrical stimulation is given in the tail of the animal (10 V, 0.3s duration with 2 s intervals), through the platform where he is. To evaluate the insulin sensitivity of the animals, there will be an insulin tolerance test "in vivo" (dose of 0.5 U insulin / kg BW). To assess the gene expression of TNF-alpha, IL-10 and NFkB is the technique of RT-PCR. Will also measured the serum lactate as a parameter of the intensity of exercises performed. The numerical results will be made through the comparison of means using ANOVA, parametric, and post-test when appropriate (Tukey or Student-Newman-Keuls). Differences between groups are considered significant when the value of P is less than 0.05.