Advanced search
Start date

Ultrastructural characterization of 5-bromo-2-deoxyuridin (BrdU) immunoreactive cells in adult ventricular and subventricular zone and its relationship with the regulating peptide CART

Grant number: 10/17519-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2011
Effective date (End): December 31, 2014
Field of knowledge:Biological Sciences - Morphology - Anatomy
Principal Investigator:II-Sei Watanabe
Grantee:Carlos Alexandre dos Santos Haemmerle
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):12/25048-3 - The cocaine-and-amphetamine related transcript-immunoreactive innervation and its relationship with neural precursors in the adult neurogenic niche of lateral ventricle, BE.EP.DR


The major niche for generation of new neurons in adult brain surrounds the lateral ventricles. In this local there is a group of cells with unique morphology and derivated from specific steps of proliferation process and cell diferentiation, that will generate the neuron. However, the description of cell that initiates the proliferation in this region is still controversial. In this way, to corroborate the ultrastructural characterization of the cells, mainly that entered in cycle of division, can contribute to solve the debate. Furthermore, the compartmentalization of periventricular region leads to a pattern of distribution for axonal fibers, as that transmit the neuroactive peptide CART, which description of its innervation and its relationship with cells of this neurogenic niche may highlight perspectives for understand the putative role of this modulator factor in the periventricular neurogenic niche. So, we aim: 1) to study the cytoarchitecture in ultrastructural level of BrdU immunoreactive (ir) cells located in periventricular region; 2) to colaborate for comprehension of tridimensional in situ organization of this region and 3) to describe the innervation of CART-ir axonal fibers and putative apositions stablished with canonical cells of periventricular region. We used Long-Evans rats and C57Bl6 and GFAP:GFP mice, adults. The animals were perfused with different fixative solutions for further ultrastructural analysis of cell morphology and neurochemistry in the periventricular region of brain, by transmission and high-resolution scanning electron microscopes, light and laser confocal. The study of proliferation at periventricular region occured after delivery of 2% arabinofuranoside (Ara-C) and the mitotic cell cycle marker BrdU (50 mg/kg, i.p.). The immunohystochemistry investigation used the primary antibodies anti-BrdU, anti-CART, anti-DCX, anti-GFAP and anti-GFP. The entire cells in periventricular region were BrdU-ir, but with variation in density of staining. We observed these occurying in ventricule linning corresponded to ependymal cells, tanycytes and astrocytes. These cells are heterogeneous in density of cillia distribution, presenting single, bi or multi-ciliated, besides be innervated in its apical surface by varicosal axons. We found the major density of CART-ir innervation in migratory path of neurons in formation and that these axons stablished apositions among neural primary progenitors, neuroblasts and cell that entered the S-phase of mitotic cycle. Therefore, 1) all cells of periventricular niche are BrdU-ir; 2) the tridimensional in situ a organization of lateral ventricle highlights the heterogeneity in density of ciliary implantation and a dense varicosal innervation apposed to the different cilia; 3) anatomical evidences suggest a role for the regulator peptide CART in periventricular neurogenesis.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS HAEMMERLE, CARLOS ALEXANDRE; NOGUEIRA, MARIA INES; WATANABE, II-SEI. The neural elements in the lining of the ventricular-subventricular zone: making an old story new by high-resolution scanning electron microscopy. FRONTIERS IN NEUROANATOMY, v. 9, . (11/19747-3, 10/17519-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
HAEMMERLE, Carlos Alexandre dos Santos. Ultrastructural characterization of 5-brome-2-deoxyuridine (BrdU) immunoreactives cells in adult ventricular and subventricular zone and its relationship with regulating peptide CART.. 2015. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

Please report errors in scientific publications list by writing to: