Photobiomodulation study on healing of skin wounds using a skin substitute of semi-interpenetrating polymer network (sIPN) as a dermal matrix in a swine model

• Grant number: 10/19852-9
• Support Opportunities: Scholarships abroad - Research
• Effective date (Start): July 01, 2011
• Effective date (End): June 30, 2012
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Jayme Adriano Farina Junior
• Grantee: Jayme Adriano Farina Junior
• Host Investigator: Weiyuan John Kao
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Research place: University of Wisconsin-Madison (UW-Madison), United States

Abstract

Large full-thickness defects of the skin resulting from burns, trauma, soft tissue, giant congenital nevi and diseases leading to skin necrosis, representing a significant clinical problem that is far from resolved. The main challenges faced are: first, there is a shortage of donor sites for autologous skin graft when the defect is greater than 50-60% of total body surface area (BSA). Second, the more conventional technique of autologous skin graft to provide coverage of defects is based on transplantation of split-thickness skin that often leads to scarring rarely discrete and irrelevant. Theoretically, these two main problems could be reduced if not eliminated, if we could develop a similar autologous full-thickness skin: the culture of a dermoepidermal skin substitute. Matrix biologically active and designed to produce a new generation of skin substitutes, both in a desired number and with a constant quality, are the guidelines of modern engineering of skin ("skingineering). Recent studies in animal models, with promising results, have been conducted at the University of Wisconsin-UW, Madison-USA with the use of a semi-interpenetrating network (sIPN) which acts as a scaffold, a biodegradable polymeric biomaterial of polyethylene glycol (PEG). Yet the future of the field biology of stem cells must be integrated in this concept and has motivated many recent researches. MSCs are multipotent cells which can give rise to mesenchymal and non-mesenchymal tissues in vitro and in vivo. The use of immortalized human dermal keratinocytes (NIKS) have demonstrated clinical efficacy in treating acute wounds (Schurr et al. 2009). Additionally, studies developed by the Center for Nanotechnology, Tissue Engineering and Photoprocessing applied to health, developed at the University of São Paulo-FFCLRP have shown the association of photobiomodulation stimulating the healing process, providing support to other positive conclusions about photodynamic therapy (PDT) and wound healing. The propose of this study is to evaluate the effects of photobiomodulation with low intensity laser (630-650 nm) on the two scaffolds of biomaterial, the photopolymerized sIPN, and the new "click" sIPN as the material platform, with addition of small molecules or proteins, and cells (MSC and NIKS) onto the platform material. In this study, it will be approached various aspects of biological skin wound healing in a swine model. (AU)