Production and immobilization of lipases produced by the endophytic fungus Cercospora kikuchii for biotechnological applications

• Grant number: 11/00743-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2011
• End date: January 31, 2014
• Field of knowledge: Engineering - Chemical Engineering - Industrial Operations and Equipment for Chemical Engineering
• Principal Investigator: Wanderley Pereira Oliveira
• Grantee: Tales Alexandre da Costa e Silva
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Enzymes are proteins presenting catalytic activity, with action in mild conditions of temperature and pressure. However, despite of the thousands of known enzymes with various activities, only some of them are used as catalysts in industrial processes. This lack of enzymatic processes on an industrial scale is the result of the scarcity of studies that give solutions to problems that arise when one proposes the design of an enzymatic reaction. The modulation of the properties of enzymes, such as penicillin G acylases by immobilization on different supports with different activation methods, and immobilization conditions can contribute to solve or mitigate the negative effects of the properties of enzymes, allowing their used in a greater number of industrial processes. The immobilization of enzymes on to solid supports using different methods (covalent bonding, adsorption, ionic bond), is a strategy often used for insolubilization and increased operational stability of enzymes, as well as for better operational control and easy retrieval of product. Thus, there is currently sought to develop methodologies to immobilize large amounts of enzyme, enable support to groups with high stability and handling it for extended periods of time. In parallel, the combination of optimization of synthesis conditions and reaction of lipases can increase its production and industrial application. This study aims to use supports, among these include agro-industrial waste, to immobilize lipases produced by the endophytic fungus Cercospora kikuchii employing drying processes, like spray drying and fluidized beds, and the characterization of the system enzyme-support for the evaluation of its biotechnological potential.

Scientific publications

(9)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

COSTA-SILVA, T. A.; CARVALHO, A. K. F.; SOUZA, C. R. F.; DE CASTRO, H. F.; BACHMANN, L.; SAID, S.; OLIVEIRA, W. P.. Immobilized enzyme-driven value enhancement of lignocellulosic-based agricultural byproducts: Application in aroma synthesis. JOURNAL OF CLEANER PRODUCTION, v. 284, FEB 15 2021. (11/00743-8)

COSTA-SILVA, T. A.; CARVALHO, A. K. F.; SOUZA, C. R. F.; FREITAS, L.; DE CASTRO, H. F.; OLIVEIRA, W. P.. Highly effective Candida rugosa lipase immobilization on renewable carriers: Integrated drying and immobilization process to improve enzyme performance. CHEMICAL ENGINEERING RESEARCH & DESIGN, v. 183, p. 15-pg., 2022-07-01. (11/00743-8)

COSTA-SILVA, T. A.; CARVALHO, A. K. F.; SOUZA, C. R. F.; DE CASTRO, H. F.; BACHMANN, L.; SAID, S.; OLIVEIRA, W. P.. Enhancement lipase activity via immobilization onto chitosan beads used as seed particles during fluidized bed drying: Application in butyl butyrate production. APPLIED CATALYSIS A-GENERAL, v. 622, JUL 25 2021. (11/00743-8)

COSTA-SILVA, T. A.; CARVALHO, A. K. F.; SOUZA, C. R. F.; DE CASTRO, H. F.; SAID, S.; OLIVEIRA, W. P.. Enzymatic Synthesis of Biodiesel Using Immobilized Lipase on a Non-commercial Support. ENERGY & FUELS, v. 30, n. 6, p. 4820-4824, JUN 2016. (11/00743-8)

COSTA-SILVA, T. A.; CARVALHO, A. K. F.; SOUZA, C. R. F.; DE CASTRO, H. F.; SAID, S.; OLIVEIRA, W. P.. Enzymatic Transesterification of Coconut Oil Using Chitosan-Immobilized Lipase Produced by Fluidized-Bed System. ENERGY & FUELS, v. 31, n. 11, p. 12209-12216, NOV 2017. (11/00743-8)

COSTA-SILVA, TALES ALEXANDRE; MARQUES, POLYANA SAMORANO; FERNANDES SOUZA, CLAUDIA REGINA; SAID, SURAIA; OLIVEIRA, WANDERLEY PEREIRA. Enzyme encapsulation in magnetic chitosan-Fe3O4 microparticles. Journal of Microencapsulation, v. 32, n. 1, p. 16-21, 2015. (11/00743-8)

COSTA-SILVA, T. A.; COGNETTE, R. C.; SOUZA, C. R. F.; SAID, S.; OLIVEIRA, W. P.. Spouted Bed Drying as a Method for Enzyme Immobilization. Drying Technology, v. 31, n. 15, p. 1756-1763, 2013. (11/00743-8)

BUFFONI CAMPOS CARNEIRO, LARA APARECIDA; COSTA-SILVA, TALES ALEXANDRE; FERNANDES SOUZA, CLAUDIA REGINA; BACHMANN, LUCIANO; OLIVEIRA, WANDERLEY PEREIRA; SAID, SURAIA. Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles. Brazilian Archives of Biology and Technology, v. 57, n. 4, p. 578-586, JUL-AUG 2014. (11/00743-8)

COSTA-SILVA, T. A.; CARVALHO, A. K. F.; SOUZA, C. R. F.; FREITAS, L.; DE CASTRO, H. F.; OLIVEIRA, W. P.; CARCEL, JA; CLEMENTE, G; GARCIAPEREZ, JV; MULET, A; et al. Immobilization of Candida rugosa lipase on eco-friendly supports by spouted-bed technology: Use in the synthesis of isoamyl caprylate. IDS'2018: 21ST INTERNATIONAL DRYING SYMPOSIUM, v. N/A, p. 8-pg., 2018-01-01. (11/00743-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Tales Alexandre da Costa e. Production and immobilization of lipases produced by the endophytic fungus Cercospora kikuchii for biotechnological applications. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.