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Production and immobilization of lipases produced by the endophytic fungus Cercospora kikuchii for biotechnological applications

Grant number: 11/00743-8
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2011
Effective date (End): January 31, 2014
Field of knowledge:Engineering - Chemical Engineering - Industrial Operations and Equipment for Chemical Engineering
Principal Investigator:Wanderley Pereira Oliveira
Grantee:Tales Alexandre da Costa e Silva
Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Enzymes are proteins presenting catalytic activity, with action in mild conditions of temperature and pressure. However, despite of the thousands of known enzymes with various activities, only some of them are used as catalysts in industrial processes. This lack of enzymatic processes on an industrial scale is the result of the scarcity of studies that give solutions to problems that arise when one proposes the design of an enzymatic reaction. The modulation of the properties of enzymes, such as penicillin G acylases by immobilization on different supports with different activation methods, and immobilization conditions can contribute to solve or mitigate the negative effects of the properties of enzymes, allowing their used in a greater number of industrial processes. The immobilization of enzymes on to solid supports using different methods (covalent bonding, adsorption, ionic bond), is a strategy often used for insolubilization and increased operational stability of enzymes, as well as for better operational control and easy retrieval of product. Thus, there is currently sought to develop methodologies to immobilize large amounts of enzyme, enable support to groups with high stability and handling it for extended periods of time. In parallel, the combination of optimization of synthesis conditions and reaction of lipases can increase its production and industrial application. This study aims to use supports, among these include agro-industrial waste, to immobilize lipases produced by the endophytic fungus Cercospora kikuchii employing drying processes, like spray drying and fluidized beds, and the characterization of the system enzyme-support for the evaluation of its biotechnological potential.

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
COSTA-SILVA, T. A.; CARVALHO, A. K. F.; SOUZA, C. R. F.; DE CASTRO, H. F.; SAID, S.; OLIVEIRA, W. P.. Enzymatic Transesterification of Coconut Oil Using Chitosan-Immobilized Lipase Produced by Fluidized-Bed System. ENERGY & FUELS, v. 31, n. 11, p. 12209-12216, . (11/00743-8)
COSTA-SILVA, TALES ALEXANDRE; MARQUES, POLYANA SAMORANO; FERNANDES SOUZA, CLAUDIA REGINA; SAID, SURAIA; OLIVEIRA, WANDERLEY PEREIRA. Enzyme encapsulation in magnetic chitosan-Fe3O4 microparticles. Journal of Microencapsulation, v. 32, n. 1, p. 16-21, . (11/00743-8)
COSTA-SILVA, T. A.; CARVALHO, A. K. F.; SOUZA, C. R. F.; DE CASTRO, H. F.; SAID, S.; OLIVEIRA, W. P.. Enzymatic Synthesis of Biodiesel Using Immobilized Lipase on a Non-commercial Support. ENERGY & FUELS, v. 30, n. 6, p. 4820-4824, . (11/00743-8)
COSTA-SILVA, T. A.; CARVALHO, A. K. F.; SOUZA, C. R. F.; DE CASTRO, H. F.; BACHMANN, L.; SAID, S.; OLIVEIRA, W. P.. Immobilized enzyme-driven value enhancement of lignocellulosic-based agricultural byproducts: Application in aroma synthesis. JOURNAL OF CLEANER PRODUCTION, v. 284, . (11/00743-8)
COSTA-SILVA, T. A.; CARVALHO, A. K. F.; SOUZA, C. R. F.; DE CASTRO, H. F.; BACHMANN, L.; SAID, S.; OLIVEIRA, W. P.. Enhancement lipase activity via immobilization onto chitosan beads used as seed particles during fluidized bed drying: Application in butyl butyrate production. APPLIED CATALYSIS A-GENERAL, v. 622, . (11/00743-8)
BUFFONI CAMPOS CARNEIRO, LARA APARECIDA; COSTA-SILVA, TALES ALEXANDRE; FERNANDES SOUZA, CLAUDIA REGINA; BACHMANN, LUCIANO; OLIVEIRA, WANDERLEY PEREIRA; SAID, SURAIA. Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles. Brazilian Archives of Biology and Technology, v. 57, n. 4, p. 578-586, . (11/00743-8)
COSTA-SILVA, T. A.; COGNETTE, R. C.; SOUZA, C. R. F.; SAID, S.; OLIVEIRA, W. P.. Spouted Bed Drying as a Method for Enzyme Immobilization. Drying Technology, v. 31, n. 15, p. 1756-1763, . (11/00743-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVA, Tales Alexandre da Costa e. Production and immobilization of lipases produced by the endophytic fungus Cercospora kikuchii for biotechnological applications. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.