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Bioanode glucose oxidase based and graphene oxide

Grant number: 11/01761-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2011
Effective date (End): August 31, 2013
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal researcher:Frank Nelson Crespilho
Grantee:Marccus Victor Almeida Martins
Home Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil


Biofuel cells (BFCs) are devices that convert bioelectrochemistry chemical energy directly into electrical energy by means of biocatalytic reactions of oxidation and reduction. In the case of BFCs type enzyme, enzymes are used in the cathode surface and/or anode and are responsible for accelerating the conversion of substrates into products from the redox process. Thus, this research project aims to develop biocell anodes enzyme applied in the fuel. For the construction of the anodes, will be using the enzyme glucose oxidase (GOx) and fructose dehydrogenase (FDH). For each type of enzyme above, biocatalytic tests will be applied in the presence of fructose. The electrochemical performance of the electrodes will be measured by cyclic voltammetry, chronoamperometry and other techniques. Aiming to achieve a better catalytic activity, will be used to support and indium tin oxide (ITO) and flexible carbon cloth (FCC) for the immobilization of enzymes mentioned above. Several methods of enzyme immobilization will be tested as cross-linking and self-assembly. The interface electrode/enzyme gold nanoparticles (AUNP) will be introduced to facilitate the transfer of electrons between the enzyme cofactor and the electrode surface. Different configurations of electrodes are applied in BCs prototypes developed in our group. Prototypes will be evaluated in the curves of power as well as the stability and lifetime of the bio cell. Finally, we intend to evaluate the applicability of these prototypes using the juice of cane sugar in natura.

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