The increasing pests cause concern since they impact production, and when uncontrolled, they lead to huge economic losses. Among major pest Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) and Heliothis virescens (Fabricius) (Lepidoptera: Noctuidae), are considered important because they attack crops like corn, cotton and tomatoes. Among the various systems used in biological pest control, the bacterium Bacillus thuringiensis stands out. This bacterium is characterized by the production of toxic proteins of several insect orders. These proteins are highly specific and this make them useful for biological control, however, this specificity allow the emergence of resistant organisms. In this sense, studies involving different insecticidal proteins of B. thuringiensis and its interactions with Brush Border Membrane Vesicles of target insects become of great importance to evaluate strategies that will delay or even avoiding the evolution of insect resistance to these proteins. Therefore, this study aims to cloning and expression of new proteins Vip3Aa42, Vip3Aa43 and Vip3Ag5 (FAPESP Proc: 2008/56622-1) in Escherichia coli, in order to analyze the correlation between the union of different Vip3A toxins and Cry1Ia10 toxin to the receptors and toxicity H. zea and H. virescens, inferring which combinations that could be used in the production of transgenic plants containing multiple genes, which are being employed to delay insect resistance to Bt toxins.
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