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Molecular techniques validation for bacterial diagnosis in fish, aiming reduction of time and cost

Grant number: 11/07951-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2011
Effective date (End): January 31, 2015
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal Investigator:Fabiana Pilarski
Grantee:Fernanda de Alexandre Sebastião
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

With the intensification of the Brazilian fish farming, characterized by increasing density storage, especially in cages, it has been observed a growing number of diseases, mainly bacterial, causing high mortality and significant economic losses in all phases of breedin. One of the problems related to the control of diseases in fish farming is the constant misuse of antibiotics and chemotherapeutics that cause problems of bacterial resistance and environmental impacts which affect the quality of the final product. Thus, the search for rapid and effective diagnostic alternative is increasingly necessary, since they contribute to control of diseases before they cause irreversible clinical signs and high mortality rates. In view of this, the molecular diagnostic techniques represented by the Polymerase Chain Reaction (PCR) and its variations, such as sequencing and real time PCR, constitute alternatives to be explored. Thus, the objectives of this work are: a) adapting the methodology of colony PCR coupled with sequencing of the 16S rRNA gene for bacteria that cause disease in fish, b) determine the sensitivity to antimicrobial florfenicol and oxytetracycline bacterial genera of higher frequency, c) validate real-time PCR assay for detection and quantification of Aeromonas hydrophila and Streptococcus agalactiae and to evaluate its sensitivity and analytical specificity d) applying the validated Methodology for the diagnosis of infections caused by these pathogens in infected experimentally tilapias groups.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SEBASTIAO, FERNANDA DE A.; LEMOS, ELIANA G. M.; PILARSKI, FABIANA. Validation of absolute quantitative real-time PCR for the diagnosis of Streptococcus agalactiae in fish. Journal of Microbiological Methods, v. 119, p. 168-175, DEC 2015. Web of Science Citations: 10.
SEBASTIAO, F. A.; FURLAN, L. R.; HASHIMOTO, D. T.; PILARSKI, F. Identification of Bacterial Fish Pathogens in Brazil by Direct Colony PCR and 16S rRNA Gene Sequencing. ADVANCES IN MICROBIOLOGY, v. 5, n. 6, p. 409-424, JUN 2015. Web of Science Citations: 17.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SEBASTIÃO, Fernanda de Alexandre. Validação de técnicas moleculares para o diagnóstico de bactérias em peixes, visando redução de tempo e custo. 2015. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Ciências Agrárias e Veterinárias..

Please report errors in scientific publications list by writing to: cdi@fapesp.br.