Myogenesis and regeneration of skeletal muscle: possible modulation by linoleic acid

• Grant number: 11/06815-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2011
• End date: September 30, 2014
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Rui Curi
• Grantee: Phablo Sávio Abreu Teixeira
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 10/02963-2 - Modulation of inflammation and insulin resistance by omega-3 fatty acids and palmitoleate, AP.TEM

Abstract

Laceration is a serious skeletal muscle injury that presents an incomplete regeneration and marked decrease in muscle strength. Skeletal muscle regeneration depends on satellite cells (muscle stem cells) activation, proliferation and differentiation to form new fibers. The purpose of the present research project is to investigate the effect of the treatment with linoleic acid upon regeneration of rat skeletal muscle. The following parameters will be evaluated: content of markers of skeletal muscle regeneration (neonatal myosin and desmin), fibrosis (vimentin and alpha-smooth actin), skeletal muscle function (strength and acute fatigue) and histological analysis of regenerated muscle. The effect of linoleic and arachidonic (a derivated-product of linoleic acid metabolism) acids upon proliferation and myogenic differentiation of muscle stem cells in vitro will be also investigated. The effect of ciclo-oxygenases (COX) inhibitors and delta-5 and delta-6 dessaturase upon myogenesis will be also evaluated. Preliminary evidences from our research group showed that fatty acids as oleic acid accelerated the myogenesis through the modulation of Notch and Beta-Catenin pathway. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ABREU, PHABLO; DIOGENES MENDES, SAVIO VICTOR; CECCATTO, VANIA MARILANDE; HIRABARA, SANDRO MASSAO. Satellite cell activation induced by aerobic muscle adaptation in response to endurance exercise in humans and rodents. Life Sciences, v. 170, p. 33-40, FEB 1 2017. (11/06815-0, 16/18633-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

TEIXEIRA, Phablo Sávio Abreu. Effect of linoleic and oleic acids on regeneration of gastrocnemius muscle after laceration in rats.. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.