Permeability study of antiretroviral drugs by in situ perfusion model. Application in the Biopharmaceutical Classification.

• Grant number: 11/09670-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2011
• End date: July 31, 2012
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Cristina Helena dos Reis Serra
• Grantee: João Batista da Silva Junior
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

HIV (Human Immunodeficiency Virus) is the AIDS (Acquired Immune Deficiency Syndrome) causative agent. Drugs such as stavudine, lamivudine and zidovudina have been used in antiretroviral therapies to minimize the effects of immune suppression in infected individuals. The drugs absorption depends on its dosage form in the environment where it is absorbed, besides physiological and physicochemical factors. Permeability studies provide knowledge that allows waiver of bioequivalence studies (biowaiver) of some drugs. The use of in situ perfusion technique can predict the drug absorption and has been widely used because of its advantages. The intestinal absorption study of antiretroviral drugs in animals can clarify some doubts in regarding to the bioavailability reported in the literature. The purpose of this study is to standardize a protocol to study the permeability by the use of technique in situ perfusion of intestinal segments of rats Wistar to correlate data obtained from animals to humans.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DEZANI, THAISA MARINHO; DEZANI, ANDRE BERSANI; DA SILVA JUNIOR, JOAO BATISTA; DOS REIS SERRA, CRISTINA HELENA. Single-Pass Intestinal Perfusion (SPIP) and prediction of fraction absorbed and permeability in humans: A study with antiretroviral drugs. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 104, p. 131-139, JUL 2016. (11/09670-3)