Effectiveness of Photodynamic Therapy using Curcumin,Photodithazine® and Chloro-aluminum phthalocyanine in inactivation of multispecies biofilm formed by Candida albicans, Candida glabrata e Streptococcus mutans: an in vitro study.
The adhesion ability of the microorganisms to different surfaces, drug resistance, as well as the interaction among the species intended to ensure the survival and proliferation of the same, resulting in lower susceptibility to these pathogens treatments and disinfection procedures. The objective of this study is to evaluate in vitro the effectiveness of antimicrobial PDT inactivation of multi-species biofilms. The biofilm will be composed of Candida albicans (ATCC 90028), Candida glabrata (ATCC 2001) and Streptococcus mutans (ATCC 25175), will be formed on acrylic resin samples. The application of PDT will be mediated by photosensitizers (SFs) Photodithazine® (PDZ), Curcumin (Cur) and Chloro-Aluminum Phthalocyanine and illuminated by the light LED. Will be used two lighting: LED 660nm (PDZ and Chloro-Aluminum Phthalocyanine) and 455nm LED (Cur) and light dose of 37.5 J/cm2. After the formation of biofilms, the resin samples will be divided according the concentration used for each FS and presence or absence of illumination by LED light. The groups will be: P-L-(positive control group: absence of FS and LED light), P-L + (control of light: absence of FS and presence of LED light), P+L- (control group FS: presence the different concentrations of each FS in the absence of LED light) and P+L+ (presence of different concentrations of FS in the presence of LED light) and then suggested treatment will be made out for each experimental group. After application of the methods, the viability of micro-organisms in the biofilm formed on the samples will be assessed by metabolic activity (XTT test), colonies count (CFU /mL), determination of total biomass (crystal violet staining) and laser scanning confocal microscopy. The results will be analyzed by appropriate statistical method.
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