Polyketide are varied secondary metabolites present in some species of micro-organisms and plants. They are part of the composition of microbial spore pigments and antibiotics, and actuan in physiological mechanisms of differentiation in the face of environmental adverse factors. They are synthesized by the enzyme complexes named Polyketide Synthases (PKSs I, II and III). Previously had carried out a prospection of new clusters for gene PKSs II in a metagenomic library of 9320 clones (FAPESP, CI process: 07/53708-0, CNPq, Master process: 130454/2010-9). An environmental insert DNA with 22,988 base pairs was found, sequenced and subjected to prediction functional of ORFs by homology and three-dimensional structure modeling "in silico". The results of these tests allowed verify the presence of homology to ABC transporter genes; Minimal PKS, cyclases; aromatases; hydroxylases, mono-oxygenases; O- methyl-transferases; carbohydrases; Peptidases; acyl transferase; LacI and LuxR genic transcription regulators, among others. There arestrong indications that it is a new pathway of polyketides pigments. The insert also contain some genes related to complex carbohydrates degradation (cellulose / chitin / lignin). It is proposed in this research project the development of experiments in genic expression (homologous and heterologous) of the biosynthetic's PKS core present in this metagenomic insert, and heterologous expression of carbohydrases of industrial interest.
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