Evaluation of the insulin signal in skeletal muscle tissue of adult rats with pulp disease

• Grant number: 11/13454-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2011
• End date: December 31, 2012
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Doris Hissako Matsushita
• Grantee: Mariane Machado Curbete
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Pathologies in the oral cavity may cause deleterious effects on several body systems. Following this line of thinking, the presence of bacteria in dental pulp may cause damage to the pulp (LP) and generate inflammation, culminating in the production of inflammatory cytokines including TNF-± and IL-6. So, cytokines may reach the systemic circulation and cause insulin resistance in important organs such as skeletal and muscle tissue. Insulin resistance is defined as a decreased of activity of insulin action in peripheral tissues, and individuals with this are predisposed to develop type 2 diabetes (T2DM). Knowing that the inflammatory cytokines TNF-± and IL-6 can cause changes in insulin signal, it becomes paramount to investigate the possibility of a local inflammatory process such as the LP "per se" cause insulin resistance in nondiabetic individuals. The objective of this project will be evaluating the degree of tyrosine phosphorylation of pp185 (IRS1/IRS2) LP in gastrocnemius skeletal muscle tissue of adults rats with LP. Will be used 30 Wistar rats (2 months old) divided into two groups: a) control rats without the LP; b) rats with LP induced in right molars employing carbon steel drill fitted with a ball on the end (0.1 mm). After 30 days of induction of LP, the animals will be anesthetized for the experiments.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ASTOLPHI, RAFAEL DIAS; CURBETE, MARIANE MACHADO; COLOMBO, NATALIA HELENA; SHIRAKASHI, DAISY JAQUELINE; CHIBA, FERNANDO YAMAMOTO; CARRARA PRIETO, ANNELISE KATRINE; ANGELO CINTRA, LUCIANO TAVARES; MOGAMI BOMFIM, SUELY REGINA; ERVOLINO, EDILSON; SUMIDA, DORIS HISSAKO. Periapical Lesions Decrease Insulin Signal and Cause Insulin Resistance. JOURNAL OF ENDODONTICS, v. 39, n. 5, p. 648-652, MAY 2013. (11/13454-4, 11/04255-8)