Physicochemical and biological properties of experimental Portland-based sealer containing different radiopacifying agents

• Grant number: 11/13573-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2011
• End date: July 31, 2014
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Marco Antonio Hungaro Duarte
• Grantee: Marina Angélica Marciano da Silva
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated scholarship(s): 13/04054-8 - Analysis of the dental discoloration with MTA and Portland cement using a bovine dental model, BE.EP.DR

Abstract

The aim of the study is to evaluate the physicochemical (radiopacity, setting time, film thickness, flow, solubility, pH, calcium ion release and dental discoloration) and biological properties of an experimental Portland-based sealer containing different concentrations of radiopacifying agents zirconium oxide and calcium tungstate. The sealers will be divided into 6 groups: I - white MTA (control), II - white Portland cement (PC), III - PC with 20% zirconium oxide, IV - PC with 50% zirconium oxide, V - PC with 20% calcium tungstate, VI - PC with 50% calcium tungstate. Physical tests will be performed in accordance to ISO 6876/2001 and ASTM C266/2008 recommendations. To determine pH and calcium ion release, 10 acrylic teeth with simulated root canals will be used for each group. The teeth with sealers will be immersed in MiliQ water and the liquid analyzed after 3, 7 and 15 days. To evaluate dental discoloration 10 bovine teeth will be cleaned and obturated with the sealers. After 7, 15, 30 and 60 days teeth will be analyzed using a spectrophotometer. For biological analysis 108 Wistar rats will be divided into 6 groups. Two polyethylene tubes filled with the sealers will be implanted in each animal. After 15, 30 and 60 days the implants with surrounding tissues will be removed. The sections will be stained with hematoxilin and eosin and analyzed under light microscope. The results will be submitted to a preliminary test of D'Agostino and Pearson to verify the presence or absence of normal distribution (p<0.05).

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARCIANO, MARINA ANGELICA; HUNGARO DUARTE, MARCO ANTONIO; CAMILLERI, JOSETTE. Calcium silicate-based sealers: Assessment of physicochemical properties, porosity and hydration. Dental Materials, v. 32, n. 2, p. E30-E40, FEB 2016. (13/04054-8, 11/13573-3)

MARCIANO, MARINA ANGELICA; HUNGARO DUARTE, MARCO ANTONIO; CAMILLERI, JOSETTE. Dental discoloration caused by bismuth oxide in MTA in the presence of sodium hypochlorite. CLINICAL ORAL INVESTIGATIONS, v. 19, n. 9, p. 2201-2209, DEC 2015. (13/04054-8, 11/13573-3)

MARCIANO, MARINA ANGELICA; CAMILLERI, JOSETTE; LIA MONDELLI, RAFAEL FRANCISCO; AMOROSO, PABLO ANDRES; CAVENAGO, BRUNO CAVALINI; PEROCHENA, ALDO DEL CARPIO; HUNGARO DUARTE, MARCO ANTONIO. Potential tooth staining due to root canal sealers containing bismuth oxide and formaldehyde. ENDO-ENDODONTIC PRACTICE TODAY, v. 9, n. 1, p. 39-45, SPR 2015. (11/13573-3, 13/04054-8)

MARCIANO, MARINA ANGELICA; ESTRELA, CARLOS; LIA MONDELLI, RAFAEL FRANCISCO; ORDINOLA-ZAPATA, RONALD; HUNGARO DUARTE, MARCO ANTONIO. Analysis of the color alteration and radiopacity promoted by bismuth oxide in calcium silicate cement. BRAZILIAN ORAL RESEARCH, v. 27, n. 4, p. 6-pg., 2013-07-01. (11/13573-3)

MARINA ANGELICA MARCIANO; CARLOS ESTRELA; RAFAEL FRANCISCO LIA MONDELLI; RONALD ORDINOLA-ZAPATA; MARCO ANTONIO HUNGARO DUARTE. Analysis of the color alteration and radiopacity promoted by bismuth oxide in calcium silicate cement. Brazilian Oral Research, v. 27, n. 4, p. 318-323, 2013-07-00. (11/13573-3)

MARCIANO, MARINA ANGELICA; COSTA, REGINALDO MENDONCA; CAMILLERI, JOSETTE; LIA MONDELLI, RAFAEL FRANCISCO; GUIMARAES, BRUNO MARTINI; HUNGARO DUARTE, MARCO ANTONIO. Assessment of Color Stability of White Mineral Trioxide Aggregate Angelus and Bismuth Oxide in Contact with Tooth Structure. JOURNAL OF ENDODONTICS, v. 40, n. 8, p. 1235-1240, AUG 2014. (13/04054-8, 12/06738-9, 11/13573-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Marina Angélica Marciano da. Physical, chemical and biological properties of an experimental Portland-based cement with different radiopacifier agents. 2014. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.