Sugarcane is one of the most important crop in Brazil, specially to ethanol´s production. Brazil is the biggest grower of sugarcane, which increase the insect pests attack. The most notably pest is the sugar cane borer, Diatraea saccharalis. This is a holometabolic insect that causes damages direct and indirect to the sugarcane crop. The control is basically done with chemical insecticides. However, these insecticides have high toxicity and high residual effects with serious consequences to the environment. Therefore, it is important search for alternative control, as the biopesticides basis on entomopathogenics microorganisms. Among, the bacterium Bacillus thuringiensis is noteworthy because produces Cry proteins with high specificity and low impact to the environment. Thus, this research aims to purify the insecticidal proteins Cry1A, Cry1Ac and Cry1Ca from a recombinant Escherichia coli and analyze the interaction, by bioassay, on a population of D. saccharalis, in order to find a control method more efficient and less harmful.
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