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Comparing anti-inflammatory and antioxidant effects of different intra-articular therapies for osteochondrosis in equines

Grant number: 11/51219-7
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): November 01, 2011
Effective date (End): October 31, 2014
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Raquel Yvonne Arantes Baccarin
Grantee:Thais Sodre de Lima Machado
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Articular diseases are fisted among the most common and important affections in the equine athlete. The use of natural antagonists or analogous synthetic products to refrain the degenerative process from progressing, as well as to promote joint homeostasis, has received increasing attention not only in equine medicine, but in other domestic animals and human beings. This study aims to compare different therapeutic modalities in respect to their anti-inflammatory and antioxidant properties, including platelet rich plasma (PRP), interleukin-1 receptor antagonist protein (IRAP II), autologous plasma (AP), hyaluronic acid (HA), and the association between HA and autologous plasma (HA+ AP) in synovial fluid cells stimulated in vitro by the addition of LPS and to verify their effect in the reestablishment of articular homeostasis in osteochondritic joints in vivo. In the initial part of the experiment synovial fluid of 12 healthy tarsocrural joints will be collected in two phases. In the first phase (anti-inflammatory evaluation) the synovial cell pool will be cultivated and subjected to different treatments. The supernatant will be connected 24 and 48 hours after the addition of LPS to determine prostaglandin E2 (PGE2), interleukin-1 (IL-1), IL-1 receptor antagonist (IL-1ra) and tumor necrosis factora (TNFα). In the second phase the antioxidant effects of different therapeutic protocols will be evaluated on synovial fluid cells challenged by LPS, through the determination of their oxidative burst. In a final step, the anti-inflammatory effects of the treatments will be evaluated in vivo, in 36 osteochondritic tarsocrural joints, that will be randomly assigned to 6 groups, according to the intra articular therapy received. Synovial fluid will be collected before arthroscopic surgery and 48 hours after the surgical procedure. Those samples will be evaluated regarding their physical characteristics, mucin precipitation clot, total and differential white cell count, total protein content determination, hyaluronic acid and chondroitin sulphate measurement and quantification of PGE2, TNF, IL-1 and IL-1ra. (AU)