|Support type:||Scholarships in Brazil - Doctorate|
|Effective date (Start):||December 01, 2011|
|Effective date (End):||May 01, 2014|
|Field of knowledge:||Agronomical Sciences - Veterinary Medicine - Animal Pathology|
|Principal Investigator:||Rosemeri de Oliveira Vasconcelos|
|Home Institution:||Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil|
Inflammation is a critical component of tumor progression and its malignancy can be correlated with the inflammatory response induced by cytokines and leukocytes infiltrating the site of tumor growth. This study aimed to characterize by immunohistochemistry the inflammatory infiltrate and pro- and anti-inflammatory cytokines in canine mammary carcinomas. To this it was made immunodetection of macrophages, lymphocytes (CD3, CD4, CD8, Treg) and cytokines (MIF, IL-10, IL-4 and TGF-² and IFN-c). Evaluating the average immunostainings by T test and analysis of variance. There was statistical difference (p <0.0001) between the control group and the group of samples from animals with simple breast carcinoma in all parameters, an increase in average animal affected by cancer. When the split occurred by histological type, all antibodies showed differences with the control group (p <0.0001). It was concluded that the simple mammary carcinomas showed an increase in macrophages count, T lymphocytes, helper T lymphocytes, Tregs as well as increased immunostaining for MIF, IL-10, IL-4 and TGF-² compared to the control group. Simple breast carcinomas solid histological pattern showed lower counts macrophages, helper T lymphocytes and Tregs, and lower immunostaining c IFN-, IL-4 and TGF-² that tubular carcinoma and papillary pattern.