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Characterization of mitochondrial gene products with unknown function in Saccharomyces Cerevisiae

Grant number: 11/18892-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2012
Effective date (End): November 11, 2016
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Mario Henrique de Barros
Grantee:Raquel Fonseca Guedes Monteiro
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Saccharomyces cerevisiae is widely used to study eukaryotic cell, and is one of the best models for the study of mitochondrial biogenesis. Being a facultative anaerobes, the isolation of mitochondrial mutants is easily obtained by the lack of growth in medium containing non-fermentable carbon sources. Many ORFs of S. cerevisiae have not been characterized yet, including several with possible mitochondrial addressing. We selected seven of this for detailed study: YDR115w and YPR116w, which show clear respiratory phenotype due to instability in the mitochondrial DNA; YDL119c, a probable mitochondrial carrier; YOR022c a possible phospholipase; YPL109c, YLR253w, YLR290c candidates to participate in the biosynthesis of coenzyme Q. To try to elucidate the function of these ORFs, we will study intracellular location and we will search proteic partners through intensification of phenotype or genetic suppressors. The possible formation of protein complexes and association of proteins will also be analyzed by sucrose gradient sedimentation.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GUEDES-MONTEIRO, RAQUEL FONSECA; FERREIRA-JUNIOR, JOSE RIBAMAR; BLEICHER, LUCAS; NOBREGA, FRANCISCO G.; BARRIENTOS, ANTONI; BARROS, MARIO H. Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits. Cell Biology International, v. 42, n. 6, SI, p. 630-642, JUN 2018. Web of Science Citations: 3.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MONTEIRO, Raquel Fonseca Guedes. Study of proteins that affect mitochondrial translation in Saccharomyces cerevisiae.. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas São Paulo.

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