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Fungal cellulase drying in spray-dryer

Grant number: 11/15990-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Engineering - Chemical Engineering - Chemical Process Industries
Principal Investigator:João Cláudio Thoméo
Grantee:Viviane Moriya Shiota
Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil


The objective of this study will give a technological treatment to enzyme extract containing cellulolytic enzymes obtained by solid state fermentation. The extract will be dried by spray-drying method and organic and inorganic supports will be applied to avoid losses during the drying process. The enzymes will be produced by the thermophilic fungus Myceliophthora sp. I-1D3, which produced large quantities of celluloses in several agro-industrial residues, and has already being done in the work of Zanelato (2011) e Shiota (2011) with the support from FAPESP (Processes MC 2008/52811-4 and IC 09/54321-7). Will be analyzed the influence of various organic and inorganic supports on the activities of CMCase and FPase extract with spray-dryer under different conditions. The enzyme powder, associated with the most efficient support after drying, will be stored under different conditions of temperature and relative humidity to evaluate the stored of these products, comparing these results with the extract. Will be evaluated the energy of inactivation, differential scanning calorimetry (DSC), the surface of particles by adsorption isotherms and particle morphology by electronic microscopy of the extracts and enzymatic powders. This project is supported by Grupo de Bioenergia do IBILCE/UNESP (GBIB), which has thematic projects within the BIOEN-FAPESP, CNPq and INCT part of Centro Paulista de Pesquisa em Bioenergia. The group has been producing fungal enzymes with high activity for various applications, such as biomass conversion to ethanol, transesterification of biodiesel and fatty acid to improve of animal feed. Thus, expect through the technological treatment to the enzymes, they become higher value-added, and the process belong potentially patentable.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SHIOTA, Viviane Moriya. Secagem de celulases de origem fúngica por spray-drying. 2014. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.

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