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Effect of phenanthrene on the gills of Prochilodus lineatus (Teleostei, Prochilodontidae) after subchronic exposure, with emphasis to chloride and mucous cells

Grant number: 11/18598-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2012
Effective date (End): December 31, 2012
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering
Principal Investigator:Marisa Narciso Fernandes
Grantee:Jakeline de Freitas Ferreira
Home Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

The phenanthrene is a polycyclic aromatic hydrocarbons present in the petroleum and its derivatives, at low concentrations it is soluble in water and persistent in the environment. Its assimilation and absorption from water by the freshwater fish occurs mainly through the gill surface and may affect the function of the organ. The main goal of the present study is to evaluate the effect of phenanthrene on the chloride and mucous cellsof the gills of Prochilodus lineatus after sub-chronic exposure to different concentrations of phenanthrene. The chloride and mucous cells will be quantified in the gill epithelium using immunohistochemistry and histochemistry, respectively. Concomitantly, the activity of Na+/K+-ATPase enzyme will be determined in the gills and the osmolality and the ion Na+, K+ e Cl- ion concentrations will be determined in plasma to evaluate the gill function in the ionic and osmotic regulation.