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Assessment of modulatory effects of rosmarinic acid and (-)- hinokinin on cellular proliferation, death and DNA repair in response to genotoxic stress on neoplastic breast cell lines

Grant number: 11/21574-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2012
Effective date (End): December 31, 2012
Field of knowledge:Biological Sciences - Genetics - Mutagenesis
Principal Investigator:Raquel Alves dos Santos
Grantee:Gabriella Machado Teixeira
Home Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil


Natural products derived-compounds (NPs) emerge as an important alternative to modulate the molecular pathways related to the genomic instability process in the early steps of carcinogenesis. Such pathways, involved in the control of cell proliferation by acting as regulators of cell cycle and apoptosis cell death, exhibit a rapid and coordinated action in response to genotoxic stress. Thus, the development of preventive approaches using NP requires the understanding of those pathways that effectively respond to such treatments. Rosmarinic acid (RA) and (-)- hinokinin (HNK) are NP-derived molecules that exhibited antigenotoxic activities, however, the mechanisms by which they exert their effects remain unknown. The present study aims to evaluate the chemopreventive potential of RA and HNK in vitro using different breast cancer-derived cell lines. The effects of RA and HNK on the doxorubicin-induced genotoxic stress will be evaluated considering the cellular pathways activated in response to DNA damage. To address these questions, cellular proliferation, cell death and DNA repair kinetics will be evaluated by bromodeoxyuridine colorimetric assay, cyto-morphological detection and comet assay, respectively.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
UZUN, NELSON; MARTINS, THOMAS DUZZI; TEIXEIRA, GABRIELLA MACHADO; CUNHA, NAYANNE LARISSA; OLIVEIRA, ROGERIO BELLE; NASSAR, EDUARDO JOSE; DOS SANTOS, RAQUEL ALVES. Poly(L-lactic acid) membranes: Absence of genotoxic hazard and potential for drug delivery. Toxicology Letters, v. 232, n. 2, p. 513-518, JAN 22 2015. Web of Science Citations: 12.

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