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Contemporary gene flow, intrapopulation spatial genetic structure and hierarchical mating system within and between fruits in a small isolated population of Genipa americana l. using microsatellite markers

Grant number: 11/01518-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2012
Effective date (End): December 31, 2014
Field of knowledge:Agronomical Sciences - Forestry Resources and Forestry Engineering
Principal Investigator:Alexandre Magno Sebbenn
Grantee:Ricardo de Oliveira Manoel
Home Institution: Faculdade de Engenharia (FEIS). Universidade Estadual Paulista (UNESP). Campus de Ilha Solteira. Ilha Solteira , SP, Brazil

Abstract

Studies of the mating system, genetic isolation of populations and the distance and the dispersal patterns of pollen and seeds are fundamentals for to understand the effects of forest fragmentation on population genetic of tree species. These studies permit to detect the effects of reproductive isolation, loss of genetic diversity and increase in the population inbreeding, as well as to define strategies for in situ conservation, germoplasm sampling for ex situ conservation and seed collect for environmental restoration. Within this contest, the aims of this project are to study by microsatellite markers the genetic diversity, the mating system, the degree of genetic isolation, distance and patterns of pollen and seed dispersal, the spatial distribution of genotypes and the effective size of a small population of Genipa americana located in a fragment of 7.2 ha. The fragment is located in Mata da Figueira, in the Experimental Station of Mogi-Guaçu, of the Forestry Institute of São Paulo. For this, all 169 adult trees existing in population will be mapped, will have the diameter at breast height (DBH) measures, leaf tissue sampled for genetic analysis and sex determination (male and female). To study the dispersal of pollen and seeds, and isolation of the population by analysis of kinship, intended to map, measure the height and samples leaves from 300 seedlings of regeneration. Open pollinated seeds will also be collected of ten trees, 50 seeds per tree in two reproductive events (2011 e 2012), being ten seeds per fruit (five fruits per tree). Seeds from the reproductive event of 2011 was already collected. To detect the effects of fragmentation on generations of descendants, the genetic diversity, inbreeding and effective size will be compared between adults, seedlings and seeds. The spatial genetic structure also will be compared between adults and seedlings in order to verify if is causing increase in kinship in the population. These results will allow understand the contemporary process of reproduction after genetic isolation by forest fragmentation and its impacts on the effective size of the generations descendants of populations of G. americana, and, thus, will serve to outline strategies for the conservation and seed collection for the environmental recovery of the population.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MANOEL, R. O.; FREITAS, M. L. M.; TAMBARUSSI, E. V.; CAMBUIM, J.; MORAES, M. L. T.; SEBBENN, A. M. Mendelian inheritance, genetic linkage, and genotypic disequilibrium at microsatellite loci in Genipa americana L. (Rubiaceae). Genetics and Molecular Research, v. 14, n. 3, p. 8161-8169, 2015. Web of Science Citations: 4.
MANOEL, R. O.; FREITAS, M. L. M.; FURLANI JUNIOR, E.; ALVES, P. F.; MORAES, M. L. T.; SEBBENN, A. M. Individual, fruit, and annual variation in correlated mating in a Genipa americana population. SILVAE GENETICA, v. 64, n. 3, p. 108-116, 2015. Web of Science Citations: 4.
MANOEL, RICARDO O.; FREITAS, MIGUEL L. M.; BARRETO, MARIANA A.; MORAES, MARIO L. T.; SOUZA, ANETE P.; SEBBENN, ALEXANDRE M. DEVELOPMENT AND CHARACTERIZATION OF 32 MICROSATELLITE LOCI IN GENIPA AMERICANA (RUBIACEAE). APPLICATIONS IN PLANT SCIENCES, v. 2, n. 3 MAR 2014. Web of Science Citations: 2.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MANOEL, Ricardo de Oliveira. Fluxo gênico, estrutura genética espacial intrapopulacional e sistema de reprodução hierárquico entre e dentro de frutos em uma pequena população isolada de Genipa americana L., utilizando marcadores microssatélites. 2014. 96 f. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Faculdade de Engenharia (Campus de Ilha Solteira)..

Please report errors in scientific publications list by writing to: cdi@fapesp.br.